PXD008175

PXD008175 is an original dataset announced via ProteomeXchange.

Title	The effect of Scp160p on polyQ-mediated protein aggregation
Description	We have previously shown that the yeast homolog of the RNA-binding vigilin proteins – Scp160p – is involved in enhancing translation efficiency in the context of codon usage. In the current study, we investigated the influence of Scp160p on the biology of polyQ reporters which differ in the codon usage of their polyQ regions. We observe that Scp160p facilitates aggregation of the polyQ reporters independent of codon usage. To explore if Scp160p might also facilitate the aggregation of endogenous polyQ-containing proteins in the yeast proteome, we combined filter trap binding and dimethyl labeling mass spectrometry to assess the aggregation state of the proteome in scp160Δ cells. Filter trap binding allows the isolation of protein aggregates which are SDS-resistant (a feature of polyQ aggregates) from wild-type and scp160Δ cells. Dimethyl labeling with nanoLC-MS/MS provided a quantitative comparison of the amount of aggregated proteins isolated by filter trap binding.
HostingRepository	PRIDE
AnnounceDate	2018-07-03
AnnouncementXML	Submission_2018-07-02_23:24:06.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nicolas Nalpas
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2017-11-09 01:17:12	ID requested
⏵ 1	2018-07-02 23:24:07	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: RNA-binding protein, polyglutamine, polyQ, Aggregation, prions, vigilin

Prof. Ralf-Peter Jansen
contact affiliation	Interfaculty Institute of Biochemistry Universität Tübingen Hoppe-Seyler Straße 4 72076 Tübingen Germany
contact email	ralf.jansen@uni-tuebingen.de
lab head
Nicolas Nalpas
contact affiliation	Tuebingen University
contact email	nalpas.nicolas@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/07/PXD008175

PRIDE project URI

• PRIDE
  1. PXD008175
     1. Label: PRIDE project
     2. Name: The effect of Scp160p on polyQ-mediated protein aggregation