PXD008174

PXD008174 is an original dataset announced via ProteomeXchange.

Title	microRNA-4331 promotes TGEV-induced mitochondrial damage via targeting RB1, up-regulating IL1RAP, and activating p38 MAPK pathway in vitro
Description	Transmissible gastroenteritis virus (TGEV), a member of the coronaviridae family, could cause fatal diarrhea of piglets and result in numerous economic losses. Previous studies demonstrated that TGEV infection could lead to mitochondrial damage and up-regulate miR-4331 level. So miR-4331 may play an important regulatory role in the control of mitochondrial function. To explore the potential role of miR-4331 in mitochondrial damage, we adopted a strategy consisting of quantitative proteomic analysis of porcine kidney (PK-15) cells in response to miR-4331 and TGEV infection. Eventually, 69 differentially expressed proteins were gained. The target of miR-4331 was identified. The effects of miR-4331 and its target RB1 on mitochondrial Ca2+ level, mitochondrial membrane potential (MMP), interleukin-1 receptor accessory protein (IL1RAP), p38 MAPK signaling pathway were investigated. The results showed that miR-4331 elevated mitochondrial Ca2+ level, reduced MMP, targets Retinoblastoma 1 (RB1), up-regulated IL1RAP, and induced activation of p38 MAPK pathway during TGEV infection. RB1 was identified as the direct targets of miR-4331 and down-regulated IL1RAP, suppressed the activation of p38 MPAK, and attenuated TGEV-induced mitochondrial damage. In addition, IL1RAP played a positive role in activating p38 MAPK signaling and negative role in TGEV-induced mitochondrial damage. The data indicate that miR-4331 aggravates TGEV-induced mitochondrial damage by repressing expression of RB1, promoting IL1RAP, and activating p38 MAPK pathway.
HostingRepository	PRIDE
AnnounceDate	2017-12-15
AnnouncementXML	Submission_2017-12-15_05:17:21.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD008174
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	suzhen huang
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-11-09 01:16:34	ID requested
⏵ 1	2017-12-15 05:17:22	announced

Zhao X, Bai X, Guan L, Li J, Song X, Ma X, Guo J, Zhang Z, Du Q, Huang Y, Tong D, . Mol Cell Proteomics, 17(2):190-204(2018) [pubmed]

curator keyword: Biological

submitter keyword: microR-4331

mitochondrial damage

proteomics

transmissible gastroenteritis virus

RB1

IL1RAP

p38 MAPK

Yong Huang
contact affiliation	College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, P.R. China
contact email	huangyong@nwsuaf.edu.cn
lab head
suzhen huang
contact affiliation	PTM
contact email	13920817975@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD008174
     1. Label: PRIDE project
     2. Name: microRNA-4331 promotes TGEV-induced mitochondrial damage via targeting RB1, up-regulating IL1RAP, and activating p38 MAPK pathway in vitro