PXD008173

PXD008173 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Proteomic profiling in mouse kidneys post-UUO
Description	Increased export of transglutaminase-2 (TG2) by tubular epithelial cells (TECs) into the surrounding interstitium modifies the extracellular homeostatic balance leading to fibrotic membrane expansion. Although silencing of extracellular TG2 ameliorates progressive kidney scarring in animal models of chronic kidney disease, the pathway through which TG2 is secreted from TECs and contributes to disease progression has not been elucidated. In this study, we developed a global proteomic approach to identify binding partners of TG2 responsible for TG2 externalization in kidneys subjected to unilateral ureteric obstruction, using TG2-knockout kidneys as negative controls. We report a robust and unbiased analysis of the membrane interactome of TG2 in fibrotic kidneys relative to the entire proteome post-UUO detected by SWATH-mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:41:58.638.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	David Boocock
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2017-11-09 01:10:11	ID requested
1	2018-02-07 13:43:36	announced
⏵ 2	2024-10-22 04:42:04	announced	2024-10-22: Updated project metadata.

Publication List

10.1681/asn.2017050479;
Furini G, Schroeder N, Huang L, Boocock D, Scarpellini A, Coveney C, Tonoli E, Ramaswamy R, Ball G, Verderio C, Johnson TS, Verderio EAM, Proteomic Profiling Reveals the Transglutaminase-2 Externalization Pathway in Kidneys after Unilateral Ureteric Obstruction. J Am Soc Nephrol, 29(3):880-905(2018) [pubmed]

10.1681/asn.2017050479;

Furini G, Schroeder N, Huang L, Boocock D, Scarpellini A, Coveney C, Tonoli E, Ramaswamy R, Ball G, Verderio C, Johnson TS, Verderio EAM, Proteomic Profiling Reveals the Transglutaminase-2 Externalization Pathway in Kidneys after Unilateral Ureteric Obstruction. J Am Soc Nephrol, 29(3):880-905(2018) [pubmed]

Keyword List

curator keyword: Biological, Biomedical
submitter keyword: SWATH, transglutaminase-2, Mouse, Chronic Kidney Disease, 6600,Kidney

curator keyword: Biological, Biomedical

submitter keyword: SWATH, transglutaminase-2, Mouse, Chronic Kidney Disease, 6600,Kidney

Contact List

David Boocock
contact affiliation	John van Geest Cancer Research Centre, School of Science and Technology, Nottingham Trent University, UK
contact email	david.boocock@ntu.ac.uk
lab head
David Boocock
contact affiliation	Nottingham Trent University
contact email	david.boocock@ntu.ac.uk
dataset submitter

Full Dataset Link List

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PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/02/PXD008173

PRIDE project URI

Repository Record List

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