PXD008171

PXD008171 is an original dataset announced via ProteomeXchange.

Title	Polylysine interactome in human cells
Description	Polylysine chains are synthesized by ribosomes when a stop codon preceding the polyA tail on the mRNA fails to terminate the translation. To clarify the faith of the polylysine-tagged chains, we analyzed polylysine interactome in the cytosol of human 293T cells. We found an extensive network of interactors involving key complexes of protein quality control, such as TRiC and 26S proteasome. The presence of components of the nuclear transport machinery among polylysine interactors and an enhanced nuclear import suggest a mechanism for compartmentalization of faulty proteins in the cell.
HostingRepository	PRIDE
AnnounceDate	2018-04-26
AnnouncementXML	Submission_2018-04-26_02:42:09.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Giulia Calloni
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-11-08 23:40:14	ID requested
⏵ 1	2018-04-26 02:42:10	announced

Lang WH, Calloni G, Vabulas RM, Polylysine is a Proteostasis Network-Engaging Structural Determinant. J Proteome Res, 17(5):1967-1977(2018) [pubmed]

curator keyword: Biological

submitter keyword: Polylysine, translation read-through, 293T cells

R. Martin Vabulas
contact affiliation	Buchmann Institute of Molecular Life Sciences and Institute of Biophysical Chemistry, Goethe University, Frankfurt am Main, Germany
contact email	vabulas@em.uni-frankfurt.de
lab head
Giulia Calloni
contact affiliation	Goethe University Frankfurt
contact email	calloni@em.uni-frankfurt.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/04/PXD008171

PRIDE project URI

• PRIDE
  1. PXD008171
     1. Label: PRIDE project
     2. Name: Polylysine interactome in human cells