PXD008168

PXD008168 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	FADD is a new protein secreted unconventionally following classical NLRP3 inflammasome activation
Description	NLRP3 inflammasome assembles in response to stress or danger signals and leads to unconventional secretion of proinflammatory IL-1. FADD is an NLRP3 inflammasome component. Here we found that classical NLRP3 inflammasome activation in human monocytes/macrophages induced FADD secretion, which required potassium efflux, functional NLRP3 sensor, ASC adaptor and caspase-1 scaffold molecule. FADD is a leaderless protein unconventionally secreted through plasma membrane-derived microvesicles. Blood-derived monocytes from rheumatoid arthritis (RA) patients secreted more FADD following NLRP3 inflammasome activation than those from healthy donors, and we found increased levels of FADD in the sera (ESPOIR cohort) and synovial fluids from RA patients. Levels of synovial FADD correlated with the inflammatory status of the joint. These data reveal that FADD secretion occurs during inflammatory disease in vivo.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:42:06.478.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	François GUILLONNEAU
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2017-11-08 04:02:16	ID requested
1	2019-02-28 04:44:29	announced
⏵ 2	2024-10-22 04:42:09	announced	2024-10-22: Updated project metadata.

Publication List

10.1038/s41419-019-1412-9;
Mouasni S, Gonzalez V, Schmitt A, Bennana E, Guillonneau F, Mistou S, Avouac J, Ea HK, Devauchelle V, Gottenberg JE, Chiocchia G, Tourneur L, The classical NLRP3 inflammasome controls FADD unconventional secretion through microvesicle shedding. Cell Death Dis, 10(3):190(2019) [pubmed]

10.1038/s41419-019-1412-9;

Mouasni S, Gonzalez V, Schmitt A, Bennana E, Guillonneau F, Mistou S, Avouac J, Ea HK, Devauchelle V, Gottenberg JE, Chiocchia G, Tourneur L, The classical NLRP3 inflammasome controls FADD unconventional secretion through microvesicle shedding. Cell Death Dis, 10(3):190(2019) [pubmed]

Keyword List

curator keyword: Biological, Biomedical
submitter keyword: human THP1 macrophages, FADD, NLRP3 inflammasome, rheumatoid arthritis,Orbitrap fusion

curator keyword: Biological, Biomedical

submitter keyword: human THP1 macrophages, FADD, NLRP3 inflammasome, rheumatoid arthritis,Orbitrap fusion

Contact List

Léa Tourneur
contact affiliation	Inserm U1016, Institut Cochin, Cnrs UMR8104, Université Paris Descartes, Sorbonne Paris Cité, Paris, France
contact email	lea.remy-tourneur@parisdescartes.fr
lab head
François GUILLONNEAU
contact affiliation	ICO Angers
contact email	francois.guillonneau@parisdescartes.fr
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/02/PXD008168
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/02/PXD008168

PRIDE project URI

Repository Record List

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