PXD008166

PXD008166 is an original dataset announced via ProteomeXchange.

Title	A comprehensive proteome-wide lysine crotonylation profiling of papaya (Carica papaya L.)
Description	Lysine crotonylation of histone proteins is a recently-identified post-translational modification with multiple cellular functions. However, lysine crotonylation of non-histone proteins in fruit cells has not yet been studied. Using high-resolution LC-MS/MS coupled with highly sensitive immune-affinity antibody analysis, a global crotonylation proteome analysis of papaya (Carica papaya L.) fruit was performed. In total, 2,120 proteins with 5,995 lysine crotonylation sites were discovered, among which eight conserved motifs were identified. Bioinformatic analysis linked crotonylated proteins to multiple metabolic pathways, including biosynthesis of antibiotics, carbon metabolism, biosynthesis of amino acids, and glycolysis. Notably, 40 crotonylated enzymes involved in various amino acid metabolism pathways were identified, suggesting a potential conserved function for crotonylation in the regulation of amino acid metabolism. Numerous crotonylation sites were identified in proteins involved in hormone signaling and cell wall-related pathways, indicating a role for crotonylation in the regulation of fruit ripening in papaya. Our comprehensive crotonylation proteome indicates diverse functions for lysine crotonylation in fruit ripening-related proteins.
HostingRepository	PRIDE
AnnounceDate	2017-12-18
AnnouncementXML	Submission_2019-03-07_05:38:49.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	zhang zhang
SpeciesList	scientific name: Carica papaya; NCBI TaxID: 3649;
ModificationList	N6-crotonyl-L-lysine
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-11-08 02:18:29	ID requested
1	2017-12-18 01:08:08	announced
⏵ 2	2019-03-07 05:38:50	announced	Updated publication reference for PubMed record(s): 29844531.

Liu K, Yuan C, Li H, Chen K, Lu L, Shen C, Zheng X, A qualitative proteome-wide lysine crotonylation profiling of papaya (Carica papaya L.). Sci Rep, 8(1):8230(2018) [pubmed]

curator keyword: Biological

submitter keyword: Amino acid metabolism

cell wall

crotonylation

fruit ripening

papaya

PTM

Kaidong Liu
contact affiliation	Life Science and Technology School, Lingnan Normal University, Zhanjiang, Guangdong 524048, PR China
contact email	liukaidong2001@126.com
lab head
zhang zhang
contact affiliation	PTM
contact email	zhang_zhang@ptm-biolab.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/12/PXD008166

PRIDE project URI

• PRIDE
  1. PXD008166
     1. Label: PRIDE project
     2. Name: A comprehensive proteome-wide lysine crotonylation profiling of papaya (Carica papaya L.)