PXD008160

PXD008160 is an original dataset announced via ProteomeXchange.

Title	Multi-laboratory analysis of sample stability for proteomics following non-cooled international transport
Description	As international collaborative research grows there is increasing desire to transport specimens around the globe. Transporting biological samples such as cells or tissues is complicated by the need to maintain integrity and minimise modification and degradation, but this is economically costly as the samples must be shipped in a frozen state. This multi-laboratory study investigated whether non-cooled transport of dried peptide samples maintained stability for proteomic analysis using mass spectrometry. Human cancer cell lysates were proteolysed and dried in Australia and shipped by air to Europe and China for label free mass spectrometry using similar LC-MS systems. Preparation and analysis of the specimens in this manner resulted in only minor differences in protein identification and high quantitative reproducibility amongst the participating laboratories. We examined any impact on peptide chemical modification and report no discrepancies compared to the starting, non-shipped sample. We conclude that the transport of non-cooled, dried peptides has little to no effect on sample integrity for downstream LC-MS analysis and therefore represents a cost-effective option to facilitate international proteomic collaborations.
HostingRepository	PRIDE
AnnounceDate	2018-03-02
AnnouncementXML	Submission_2018-03-02_00:28:11.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pascal Steffen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-11-07 23:45:03	ID requested
⏵ 1	2018-03-02 00:28:13	announced

Steffen P, Krisp C, Yi W, Yang P, Molloy MP, Schl, ü, ter H, Multi-laboratory analysis of the variability of shipped samples for proteomics following non-cooled international transport. Anal Biochem, 548():60-65(2018) [pubmed]

curator keyword: Biomedical

submitter keyword: Proteomics, peptides, stability, LC-MS, Multi-laboratory

Hartmut Schlueter
contact affiliation	Institute of Clinical Chemistry/Central Laboratories, Mass Spectrometric Proteomics, University Clinic Hamburg-Eppendorf
contact email	hschluet@uke.de
lab head
Pascal Steffen
contact affiliation	University Clinic Hamburg-Eppendorf, Germany Macquarie University/ APAF Sydney, Australia
contact email	p.steffen@uke.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD008160
     1. Label: PRIDE project
     2. Name: Multi-laboratory analysis of sample stability for proteomics following non-cooled international transport