<<< Full experiment listing

PXD008156

PXD008156 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHuman myocardial ischemia/reperfusion injury model to study cardiac stem cells activation and regeneration mechanisms
DescriptionThe importance of regenerative potential of cardiac stem/progenitor cells (CSCs) after acute myocardial infarction (AMI) has been emphasized in the last decade. Several studies have demonstrated that this cell population has a relevant role in myocardial repair, supported by the establishment of a paracrine cross-talk between CSCs and the injured tissue. However, the majority of current in vitro strategies to study these cells fails to include important modulators of CSC activity, such as the paracrine effect of cardiomyocytes (CMs) and the physicochemical changes occurring in tissue during Ischemia/Reperfusion (I/R) injury. In this work, we established the first human in vitro heterotypic model of myocardial I/R injury with human CSCs (hCSCs) and human induced pluripotent cell-derived cardiomyocytes (hiPSC-CMs) using transwell inserts. hCSCs response to I/R was characterized using advanced whole proteomic mass spectrometry-based tools that allowed us to propose new pathways in hCSCs regenerative process, including: cell cycle regulation inhibition, proliferation through EGF signaling, gluthathione-mediated reactive oxygen species detoxification, and signaling through several paracrine growth factors. The model established in this work contributes with new insights regarding hCSC biology in response to AMI and provides an important tool to study and modulate molecular mechanisms involved in the regenerative potential of CSCs.
HostingRepositoryPRIDE
AnnounceDate2019-02-18
AnnouncementXMLSubmission_2019-06-27_06:41:28.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMaria Sebastião
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentTripleTOF 6600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-11-07 07:44:39ID requested
12019-02-18 07:23:57announced
22019-06-27 06:46:30announcedUpdated publication reference for PubMed record(s): 30845956.
Publication List
Sebasti, ã, o MJ, Serra M, Pereira R, Palacios I, Gomes-Alves P, Alves PM, Human cardiac progenitor cell activation and regeneration mechanisms: exploring a novel myocardial ischemia/reperfusion in vitro model. Stem Cell Res Ther, 10(1):77(2019) [pubmed]
Keyword List
curator keyword: Biological, Biomedical
submitter keyword: cardiac stem/progenitor cells
acute myocardial infarction
ischemia/reperfusion injury
Contact List
Paula Marques Alves
contact affiliationITQB-NOVA, Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Av. Da República, 2780-157 Oeiras, Portugal;iBET, Instituto de Biologia Experimental e Tecnológica, Apartado 12, 2780-901, Oeiras , Portugal
contact emailmarques@ibet.pt
lab head
Maria Sebastião
contact affiliationiBET/ ITQB-NOVA
contact emailmsebastiao@itqb.unl.pt
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/02/PXD008156
PRIDE project URI
Repository Record List
[ + ]