PXD008150

PXD008150 is an original dataset announced via ProteomeXchange.

Title	Cell shape information is transduced through tension-independent mechanisms
Description	The shape of a cell within tissues can represent the history of chemical and physical signals that it encounters, but can information from cell shape regulate cellular phenotype independently? Using optimal control theory to constrain reaction-diffusion schemes that are dependent on different surface-to-volume relationships, we find that information from cell shape can be resolved from mechanical signals. We used microfabricated 3-D biomimetic chips to validate predictions that shape-sensing occurs in a tension-independent manner through β3 integrin signaling pathway in human kidney podocytes and smooth muscle cells. Differential proteomics and functional ablation assays indicate that β3 integrin is critical in transduction of shape signals through ezrin-radixin-moesin (ERM) family. We used experimentally determined diffusion coefficients and experimentally validated simulations to show that shape sensing is an emergent cellular property enabled by multiple molecular characteristics of β3 integrin. We conclude that 3-D cell shape information, transduced through tension-independent mechanisms, can regulate phenotype.
HostingRepository	PRIDE
AnnounceDate	2018-07-26
AnnouncementXML	Submission_2018-07-26_06:31:49.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD008150
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Tong Liu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Ammonia-loss; Dehydrated; Oxidation; Acetyl; Carbamidomethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-11-07 02:10:09	ID requested
⏵ 1	2018-07-26 06:31:51	announced

Ron A, Azeloglu EU, Calizo RC, Hu M, Bhattacharya S, Chen Y, Jayaraman G, Lee S, Neves-Zaph SR, Li H, Gordon RE, He JC, Hone JC, Iyengar R, Cell shape information is transduced through tension-independent mechanisms. Nat Commun, 8(1):2145(2017) [pubmed]

curator keyword: Biological

submitter keyword: LC-MS/MS

Ravi Iyengar
contact affiliation	Icahn School of Medicine at Mount Sinai Department of Pharmacological Sciences New York, NY 10029
contact email	ravi.iyengar@mssm.edu
lab head
Tong Liu
contact affiliation	Rutgers University
contact email	linto@njms.rutgers.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/07/PXD008150

PRIDE project URI

• PRIDE
  1. PXD008150
     1. Label: PRIDE project
     2. Name: Cell shape information is transduced through tension-independent mechanisms