PXD008120

PXD008120 is an original dataset announced via ProteomeXchange.

Title	Dengue Virus NS1 Replicon - Dengue virus (DENV) is a major human pathogen that belongs to the flavivirus genus
Description	Dengue virus (DENV) is a major human pathogen that belongs to the flavivirus genus. Among non-structural viral proteins, NS1 is an enigmatic factor that is exclusively encoded by members of the flavivirus genus within the Flaviviridae family and accomplishes different functions during DENV infection. To gain insight into the molecular and cellular function of NS1 in viral replication, we generated a tagged NS1 DENV replicon and identified the associated host proteins during active viral replication. Replicons are self-replicating flavivirus RNAs containing large in-frame deletions in the structural genes and are useful tools to study translation and RNA amplification of several flaviviruses. To establish a global map of NS1-host protein interactions occurring during DENV replication, we stably expressed a DENV2 replicon encoding NS1 tagged with N-terminal FLAG and HA epitopes (FH-NS1) or the untagged version (WT) in three different human cell lines (Raji, HeLa and HAP1). Interactors of NS1 were identified by AP-MS/MS from these three different cell lines.
HostingRepository	PRIDE
AnnounceDate	2020-03-12
AnnouncementXML	Submission_2020-03-12_03:42:44.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pierre-Olivier Vidalain
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-11-03 03:36:33	ID requested
1	2017-11-29 03:05:42	announced
⏵ 2	2020-03-12 03:42:44	announced	2020-03-12: Updated project metadata.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Dengue Virus, NS1, Human cells, Replicon

Ali Amara
contact affiliation	Institut National de la Sant?? et de la Recherche M??dicale U944, Centre National de la Recherche Scientifique Unit?? Mixte de Recherche 7212, Institut Universitaire d???H??matologie, Sorbonne Paris Cit??, Universit?? Paris Diderot, H??pital Saint Louis, 1 Avenue Claude Vellefaux, 75010 Paris, France
contact email	ali.amara@inserm.fr
lab head
Pierre-Olivier Vidalain
contact affiliation	Universit?? Paris Descartes
contact email	pierre-olivier.vidalain@parisdescartes.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD008120

PRIDE project URI

• PRIDE
  1. PXD008120
     1. Label: PRIDE project
     2. Name: Dengue Virus NS1 Replicon - Dengue virus (DENV) is a major human pathogen that belongs to the flavivirus genus