PXD008119

PXD008119 is an original dataset announced via ProteomeXchange.

Title	A Comprehensive Proteomics Study on Platelet Concentrates: Platelet Proteome, Storage Time and Mirasol Pathogen Reduction Technology
Description	Platelet concentrates (PCs) represent a blood transfusion product with a major concern for safety as their storage temperature (20-24ºC) allows bacterial growth, and their maximum storage time period (less than a week) precludes complete microbiological testing. Pathogen inactivation technologies (PITs) provide an additional layer of safety to the blood transfusion products from known and unknown pathogens (such as bacteria, viruses and parasites). In this context, PITs (such as Mirasol Pathogen Reduction Technology -PRT-) have been developed and are implemented in many countries. However, several studies have shown in vitro that Mirasol PRT induces a certain level of platelet shape change, hyperactivation, basal degranulation and increased oxidative damage during storage. It has been suggested that Mirasol PRT might accelerate what has been described as the platelet storage lesion (PSL), but supportive molecular signatures have not been obtained. We aimed at dissecting the influence of both variables, i.e. Mirasol PRT and storage time, at the proteome level. We present comprehensive proteomics data analysis of Control PCs and PCs treated with Mirasol PRT at storage days 2, 6 and 8. Our workflow was set to perform proteomics analysis using a gel-free and label-free quantification (LFQ) approach. Semi-quantification was based on LFQ signal intensities of identified proteins using MaxQuant/Perseus software platform. We identified marginal differences between Mirasol PRT and control PCs during storage. However, those significant changes at the proteome level were specifically related to the functional aspects previously described to affect platelets upon Mirasol PRT, and in addition, the effect of Mirasol PRT on the platelet proteome appeared not to be exclusively due to an accelerated or enhanced PSL. In summary, semi-quantitative proteomics allows to discern between proteome changes due to Mirasol PRT or PSL, and proves to be a methodology suitable to phenotype platelets in an unbiased manner, in various physiological contexts.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:41:32.151.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Laura Gutiérrez
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2017-11-03 01:24:13	ID requested
1	2018-03-23 05:46:54	announced
⏵ 2	2024-10-22 04:41:40	announced	2024-10-22: Updated project metadata.

10.1080/09537104.2018.1447658;

Salunkhe V, De Cuyper IM, Papadopoulos P, van der Meer PF, Daal BB, Villa-Fajardo M, de Korte D, van den Berg TK, Guti, é, rrez L, A comprehensive proteomics study on platelet concentrates: Platelet proteome, storage time and Mirasol pathogen reduction technology. Platelets, 30(3):368-379(2019) [pubmed]

curator keyword: Technical, Biological

submitter keyword: Platelet Function Tests, Storage Time,Platelet Transfusion, Pathogen Inactivation Technologies, Blood Safety, Proteomics

Laura Gutiérrez
contact affiliation	Hospital Clínico San Carlos, Instituto de Investigación Sanitaria San Carlos (IdISSC), Dept. Hematology, Megakaryopoiesis and Platelets Group, Madrid, Spain
contact email	lgutierrezg@salud.madrid.org
lab head
Laura Gutiérrez
contact affiliation	Instituto de Investigación Sanitaria del Principado de Asturias (ISPA)
contact email	l.gutierrez@me.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD008119
     1. Label: PRIDE project
     2. Name: A Comprehensive Proteomics Study on Platelet Concentrates: Platelet Proteome, Storage Time and Mirasol Pathogen Reduction Technology