PXD008087

PXD008087 is an original dataset announced via ProteomeXchange.

Title	PROTEOME OF BOVINE MITOCHONDRIA AND ROD OUTER SEGMENT DISKS: COMMONALITIES AND DIFFERENCES.
Description	The retinal rod outer segment (OS) isa stack of diskssurrounded by the plasma membrane,housing proteins related to phototransduction as well asmitochondrial proteins involved in oxidative phosphorylation (OxPhos). This prompted us to compare the proteome of bovine OS disks and mitochondria, to assess the significant top gene signatures of each sample.The two proteomes, obtained byLTQ-OrbitrapVelos mass spectrometry, were compared by statistical analyses. 4139 proteins were identified, 2045 of which overlapping in the two sets. Non-hierarchical Spearman’s correlogram revealed that the groups were clearly discriminated. Partial Least Square Discriminant plus Support Vector Machine analysis identified the major discriminative proteins, implied in photo-transduction and lipid metabolism, respectively. Gene ontology analysis identified top gene signatures of disk proteome, enriched in vesiculation, glycolysis, and OxPhos proteins. The tricarboxylic acid (TCA) cycle and the electron transport proteinswere similarly enriched in the two samples: Notably thelatterwas up regulated in disks.Data suggest that the OxPhos proteins may derive from mitochondria becoming a true OS component, and acquiring different functionality once in ectopic location. Understanding theyet unanswered issue of chemical energy supply in the rod OS, can positively influence our vision of retinal physiology and pathology.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:49:33.318.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chiara Lavarello
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-10-31 06:32:14	ID requested
1	2022-03-01 10:02:42	announced
⏵ 2	2023-11-14 08:49:33	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: ATP synthase

disks;electron transfer chain

mass spectrometry

mitochondria

oxidative phosphorylation

Rod Outer Segment

transmission electron microscopy.

Andrea Petretto
contact affiliation	Istituto Gaslini
contact email	andreapetretto@gaslini.org
lab head
Chiara Lavarello
contact affiliation	Istituto G.Gaslini
contact email	chiaralavarello@ospedale-gaslini.ge.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD008087
     1. Label: PRIDE project
     2. Name: PROTEOME OF BOVINE MITOCHONDRIA AND ROD OUTER SEGMENT DISKS: COMMONALITIES AND DIFFERENCES.