PXD008081

PXD008081 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomics reveals proteins involved in the progression from non-cancerous lesions to gastric cancer
Description	Gastric cancer (GC) is among the most aggressive malignancy affecting the world population. With almost a million cases remains in fifth position in incidence, while reaching the third position in mortality. The progression of GC is slow with several prolonged and sequential precancerous stages, including chronic gastritis, intestinal metaplasia, dysplasia and finally gastric cancer. Here we used the iTRAQ chemistry in combination with high-resolution mass spectrometry analysis to describe, from the proteomics point of view, the progression of the GC disease. Tissue samples from three stages: chronic gastritis, intestinal metaplasia and gastric adenocarcinoma, were selected for the quantitative proteomics analysis. From four independent replicates we identified and reported quantitative data for 3,914 different proteins with at least two unique peptides confidently quantified in all replicates. We uncovered pathways and processes dysregulated between the different stages. The initial transformation is characterized by the down-regulation of ribosomes and the protein processing in the endoplasmic reticulum, while overexpressing cell survival pathway and proteins such as GSTP1 and EPCAM. The transformation to GC involved the activation of DNA replication and Spliceosome pathways, overexpression of proteins supporting high rates of proliferation such as NPM1. In GC SIRT3 and SIRT5 were down-regulated, which correlated with the impairment of the mitochondrial pathways and overexpression of enzymes supporting the glycolytic phenotype such as HK3 and PCK2. Several proteins found dysregulated between stages of the progression of GC has potential to be used as specific biomarkers and/or targets for therapeutic treatment.
HostingRepository	PRIDE
AnnounceDate	2019-09-27
AnnouncementXML	Submission_2019-09-27_11:57:55.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jeovanis Gil
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-10-31 02:24:21	ID requested
⏵ 1	2019-09-27 11:57:56	announced

Fern, á, ndez-Coto DL, Gil J, Hern, á, ndez A, Herrera-Goepfert R, Castro-Romero I, Hern, á, ndez-M, á, rquez E, Arenas-Linares AS, Calderon-Sosa VT, Sanchez-Aleman M Á, Mendez-Tenorio A, Encarnaci, ó, n-Guevara S, Ayala G, Quantitative proteomics reveals proteins involved in the progression from non-cancerous lesions to gastric cancer. J Proteomics, 186():15-27(2018) [pubmed]

curator keyword: Biomedical

submitter keyword: Gastric cancer, iTRAQ labelling, Quantitative proteomics, Cancer progression, intestinal metaplasia, chronic gastritis

Guadalupe Ayala Aguilar
contact affiliation	Departamento de interacción epidemiológica, Instituto Nacional de Salud Pública. Universidad No. 655, Col. Santa María Ahuacatitlán, Cuernavaca, Morelos. CP 62100. México.
contact email	gayala@insp.mx
lab head
Jeovanis Gil
contact affiliation	CCG-UNAM
contact email	jeovanis@ccg.unam.mx
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/09/PXD008081

PRIDE project URI

• PRIDE
  1. PXD008081
     1. Label: PRIDE project
     2. Name: Quantitative proteomics reveals proteins involved in the progression from non-cancerous lesions to gastric cancer