PXD008079

PXD008079 is an original dataset announced via ProteomeXchange.

Title	Integrated proteomics reveals apoptosis-related mechanisms associated with past-chronic stage of placental malaria
Description	Malaria in pregnancy remains a substantial public health concern in malaria-endemic areas. Accumulation of maternal immune cells in the placenta and increased levels of inflammatory cytokines caused by sequestration of Plasmodium falciparum-infected erythrocytes (IE) in the placental intervillous blood spaces have been associated to poor neonatal outcomes, including low birth weight due to fetal growth restriction. However, little is known about the molecular changes occurring in the placenta in past-stages of P. falciparum infection when the hemozoin pigment is present in the absence of parasites. We conducted an integrated proteome, phosphoproteome and glycoproteome analysis in P. falciparum infected and non-infected placentas aiming to find molecular changes occurring in past-stage infection. A total of 2946 proteins, 1733 glycosites and 4100 phosphosites were identified and quantified in this study, disclosing overrepresented processes related to oxidative stress, protein folding and regulation of apoptosis, as well as AKT and ERK signaling pathways activation, which together with clinical data and literature-based information were further correlated to an increased apoptosis in infected placentas. This study showed apoptosis-related mechanisms associated with past-stage of malaria infection that can be further explored as therapeutic target against adverse pregnancy outcomes in placental malaria.
HostingRepository	PRIDE
AnnounceDate	2018-09-25
AnnouncementXML	Submission_2018-09-25_08:22:18.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rebeca Sakuma
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-10-31 02:15:08	ID requested
⏵ 1	2018-09-25 08:22:20	announced

Kawahara R, Rosa-Fernandes L, Dos Santos AF, Bandeira CL, Dombrowski JG, Souza RM, Da Fonseca MP, Festuccia WT, Labriola L, Larsen MR, Marinho CRF, Palmisano G, Integrated Proteomics Reveals Apoptosis-related Mechanisms Associated with Placental Malaria. Mol Cell Proteomics, 18(2):182-199(2019) [pubmed]

curator keyword: Biomedical

submitter keyword: proteome, phosphoproteome, glycoproteome, placental malaria, P. falciparum

Giuseppe Palmisano
contact affiliation	Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo São Paulo, Brazil
contact email	palmisano.gp@gmail.com
lab head
Rebeca Sakuma
contact affiliation	University of Sao Paulo
contact email	rebecasakuma@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/09/PXD008079

PRIDE project URI

• PRIDE
  1. PXD008079
     1. Label: PRIDE project
     2. Name: Integrated proteomics reveals apoptosis-related mechanisms associated with past-chronic stage of placental malaria