PXD008073

PXD008073 is an original dataset announced via ProteomeXchange.

Title	Human follicular fluid proteomic and peptidomic composition quantitative studies by SWATH-MS methodology. Applicability of high pH RP-HPLC fractionation
Description	Analysis of proteomic composition of human follicular fluid (hFF) has been proposed as a potential tool of oocyte quality evaluation. In order to develop an efficient method to investigate the hFF proteome and peptidome components, we applied a prefractionation scheme of hFF material consisting of ultrafiltration, optional immunodepletion, and high pH RP-HPLC separation. Resulting from ultrafiltration Low Molecular-Weight Fraction peptides (LMWF, <10 kDa) and High Molecular-Weight Fraction proteins (HMWF, >10 kDa) were processed, measured, and analyzed separately. We identified 302 proteins in HMWF and 161 proteins in LMWF in all qualitative experiments. Combining data from single fractionation experiments we obtained and compared 18 spectral libraries (10 for HMWF, 8 for LMWF). We conducted quantitative SWATH-MS measurements of pool hFF samples for 108 HMWF proteins and 250 LMWF peptides. HMWF proteomic libraries were of similar quality with a few exceptions, even when comparing most basic and complex libraries. All LMWF peptidomic libraries turned out to be of poor quantification quality, however they enabled measurement of higher numbers of peptides with increasing input of experiment data, in contrast to proteomic libraries.
HostingRepository	PRIDE
AnnounceDate	2018-03-19
AnnouncementXML	Submission_2018-03-19_03:21:11.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Aleksandra Lewandowska
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2017-10-30 02:54:34	ID requested
⏵ 1	2018-03-19 03:21:12	announced

Lewandowska AE, Macur K, Czaplewska P, Liss J, Ł, ukaszuk K, O, ł, dziej S, Human follicular fluid proteomic and peptidomic composition quantitative studies by SWATH-MS methodology. Applicability of high pH RP-HPLC fractionation. J Proteomics, 191():131-142(2019) [pubmed]

curator keyword: Technical, Biological

submitter keyword: Human, Follicular Fluid, SWATH-MS, fractionation

Stanislaw Oldziej
contact affiliation	Laboratory of Biopolymer Structure, Intercollegiate Faculty of Biotechnology UG-MUG, University of Gdansk, Gdansk, Poland
contact email	stanislaw.oldziej@biotech.ug.edu.pl
lab head
Aleksandra Lewandowska
contact affiliation	University of Gdansk
contact email	aleksandra.lewandowska@biotech.ug.edu.pl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD008073
     1. Label: PRIDE project
     2. Name: Human follicular fluid proteomic and peptidomic composition quantitative studies by SWATH-MS methodology. Applicability of high pH RP-HPLC fractionation