PXD008069

PXD008069 is an original dataset announced via ProteomeXchange.

Title	Proteomic changes in Oryza sativa leaves in response to MSP1 and flg22 treatments
Description	Rice blast disease caused by Magnaporthe oryzae is one of the most damaging diseases affecting rice productivity. Previously, we reported a novel M. oryzae- secreted protein MSP1, which triggers cell death and pathogen-associated molecular pattern (PAMP)-triggered immune (PTI) responses in rice. To investigate the MSP1 induced defense response in rice at the protein level, we employed a label-free quantitative proteomic approach, in parallel with the flg22, which is a wellknown elicitor. Proteomics analysis using the MaxQuant-Perseus platform led to the identification of 4087 proteins of which 417 showed significant differences (multiple sample test, ANOVA p<0.05) in response to MSP1 and/or flg22 treatments. Functional annotation of the differential proteins showed that proteins related to the primary metabolism, secondary metabolism and lipid metabolism were strongly down-regulated, while elevated proteins were mainly associated with the stress response, chromatin remodeling, post-translational modification of proteins and signaling.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:35:02.988.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Iris Finkemeier
SpeciesList	scientific name: Oryza sativa (Rice); NCBI TaxID: 4530;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-10-30 02:29:23	ID requested
1	2018-07-06 00:27:23	announced
⏵ 2	2024-10-22 04:35:10	announced	2024-10-22: Updated project metadata.

10.1016/j.jprot.2018.04.015;

Meng Q, Gupta R, Min CW, Kim J, Kramer K, Wang Y, Park SR, Finkemeier I, Kim ST, A proteomic insight into the MSP1 and flg22 induced signaling in Oryza sativa leaves. J Proteomics, 196():120-130(2019) [pubmed]

curator keyword: Biological

submitter keyword: MSP1, label-free quantitative proteomics, Magnaporthe oryzae, PAMP-triggered immunity,Rice

Iris Finkemeier
contact affiliation	Max Planck Institute for Plant Breeding Research Plant Proteomics Group Carl von Linné Weg 10 50829 Cologne Germany
contact email	iris.finkemeier@uni-muenster.de
lab head
Iris Finkemeier
contact affiliation	University of Muenster
contact email	iris.finkemeier@uni-muenster.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/07/PXD008069

PRIDE project URI

• PRIDE
  1. PXD008069
     1. Label: PRIDE project
     2. Name: Proteomic changes in Oryza sativa leaves in response to MSP1 and flg22 treatments