PXD008067

PXD008067 is an original dataset announced via ProteomeXchange.

Title	A dual inhibitor of Staphylococcus aureus virulence and biofilm attenuates expression of major toxins and adhesins
Description	Staphylococcus aureus is a major bacterial pathogen that invades and damages host tissue by the expression of devastating toxins. We here performed a phenotypic screen of 35 molecules that were structurally inspired by previous hydroxyamide-based S. aureus virulence inhibitors compiled from commercial sources or designed and synthesized de novo. One of the most potent compounds, AV73, did not only reduce hemolytic alpha-hemolysin expression in S. aureus but also impeded in vitro biofilm formation. The effect of AV73 on bacterial proteomes and extracellular protein levels were analyzed by quantitative proteomics and revealed a significant down-regulation of major virulence and biofilm promoting proteins. To elucidate the mode of action of AV73, target identification was performed using affinity-based protein profiling (AfBPP).
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:40:59.427.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Barbara Hofbauer
SpeciesList	scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2017-10-30 02:25:27	ID requested
1	2018-03-05 00:01:36	announced
⏵ 2	2024-10-22 04:41:06	announced	2024-10-22: Updated project metadata.

Hofbauer B, Vomacka J, Stahl M, Korotkov VS, Jennings MC, Wuest WM, Sieber SA, Dual Inhibitor of Staphylococcus aureus Virulence and Biofilm Attenuates Expression of Major Toxins and Adhesins. Biochemistry, 57(11):1814-1820(2018) [pubmed]

10.1021/acs.biochem.7b01271;

curator keyword: Biomedical

submitter keyword: virulence, proteomics,biofilm, target identification, phenotype

Stephan Axel Sieber
contact affiliation	Department of Chemistry, Technische Universität München, Germany
contact email	stephan.sieber@mytum.de
lab head
Barbara Hofbauer
contact affiliation	Technische Universität München
contact email	b.hofbauer@tum.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD008067
     1. Label: PRIDE project
     2. Name: A dual inhibitor of Staphylococcus aureus virulence and biofilm attenuates expression of major toxins and adhesins