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PXD008056

PXD008056 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEndogenous androgen receptor proteomic profiling reveals genomic subcomplex involved in prostate tumorigenesis
DescriptionAndrogen receptor (AR) is a key player in prostate cancer development and progression. Here we applied immunoprecipitation mass spectrometry of endogenous AR in LNCaP cells to identify components of the AR transcriptional complex. In total, 66 known and novel AR interactors were identified in the presence of synthetic androgen, most of which were critical for AR-driven prostate cancer cell proliferation. A subset of AR interactors required for LNCaP proliferation were profiled using chromatin immunoprecipitation assays followed by sequencing, identifying distinct genomic subcomplexes of AR interaction partners. Interestingly, three major subgroups of genomic subcomplexes were identified, where selective gain of function for AR genomic action in tumorigenesis was found, dictated by FOXA1 and HOXB13. In summary, by combining proteomic and genomic approaches we reveal subclasses of AR transcriptional complexes, differentiating normal AR behavior from the oncogenic state. In this process, the expression of AR interactors has key roles by reprogramming the AR cistrome and interactome in a genomic location-specific manner.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:41:26.105.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLiesbeth Hoekman
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-10-26 06:12:59ID requested
12017-10-27 06:39:18announced
22017-11-01 06:20:33announcedUpdated publication reference for PubMed record(s): 28925401.
32024-10-22 04:41:34announced2024-10-22: Updated project metadata.
Publication List
Stelloo S, Nevedomskaya E, Kim Y, Hoekman L, Bleijerveld OB, Mirza T, Wessels LFA, van Weerden WM, Altelaar AFM, Bergman AM, Zwart W, Endogenous androgen receptor proteomic profiling reveals genomic subcomplex involved in prostate tumorigenesis. Oncogene, 37(3):313-322(2018) [pubmed]
10.1038/onc.2017.330;
Keyword List
curator keyword: Biological, Biomedical
submitter keyword: Prostate cancer, Interactome,RIME, Proteomics, Androgen Receptor
Contact List
A. F. Maarten Altelaar
contact affiliationMass Spectrometry and Proteomics Facility, The Netherlands Cancer Institute, Amsterdam, The Netherlands
contact emailm.altelaar@nki.nl
lab head
Liesbeth Hoekman
contact affiliationThe Netherlands Cancer Institute, Amsterdam, The Netherlands.
contact emaill.hoekman@nki.nl
dataset submitter
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Dataset FTP location
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PRIDE project URI
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