PXD008056 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Endogenous androgen receptor proteomic profiling reveals genomic subcomplex involved in prostate tumorigenesis |
Description | Androgen receptor (AR) is a key player in prostate cancer development and progression. Here we applied immunoprecipitation mass spectrometry of endogenous AR in LNCaP cells to identify components of the AR transcriptional complex. In total, 66 known and novel AR interactors were identified in the presence of synthetic androgen, most of which were critical for AR-driven prostate cancer cell proliferation. A subset of AR interactors required for LNCaP proliferation were profiled using chromatin immunoprecipitation assays followed by sequencing, identifying distinct genomic subcomplexes of AR interaction partners. Interestingly, three major subgroups of genomic subcomplexes were identified, where selective gain of function for AR genomic action in tumorigenesis was found, dictated by FOXA1 and HOXB13. In summary, by combining proteomic and genomic approaches we reveal subclasses of AR transcriptional complexes, differentiating normal AR behavior from the oncogenic state. In this process, the expression of AR interactors has key roles by reprogramming the AR cistrome and interactome in a genomic location-specific manner. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:41:26.105.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Liesbeth Hoekman |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-10-26 06:12:59 | ID requested | |
1 | 2017-10-27 06:39:18 | announced | |
2 | 2017-11-01 06:20:33 | announced | Updated publication reference for PubMed record(s): 28925401. |
⏵ 3 | 2024-10-22 04:41:34 | announced | 2024-10-22: Updated project metadata. |
Publication List
Stelloo S, Nevedomskaya E, Kim Y, Hoekman L, Bleijerveld OB, Mirza T, Wessels LFA, van Weerden WM, Altelaar AFM, Bergman AM, Zwart W, Endogenous androgen receptor proteomic profiling reveals genomic subcomplex involved in prostate tumorigenesis. Oncogene, 37(3):313-322(2018) [pubmed] |
10.1038/onc.2017.330; |
Keyword List
curator keyword: Biological, Biomedical |
submitter keyword: Prostate cancer, Interactome,RIME, Proteomics, Androgen Receptor |
Contact List
A. F. Maarten Altelaar |
contact affiliation | Mass Spectrometry and Proteomics Facility, The Netherlands Cancer Institute, Amsterdam, The Netherlands |
contact email | m.altelaar@nki.nl |
lab head | |
Liesbeth Hoekman |
contact affiliation | The Netherlands Cancer Institute, Amsterdam, The Netherlands. |
contact email | l.hoekman@nki.nl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/10/PXD008056 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD008056
- Label: PRIDE project
- Name: Endogenous androgen receptor proteomic profiling reveals genomic subcomplex involved in prostate tumorigenesis