PXD008055

PXD008055 is an original dataset announced via ProteomeXchange.

Title	The global acetylome of the human pathogen Vibrio cholerae V52 reveals lysine acetylation of major transcriptional regulators
Description	Protein lysine acetylation is recognized as an important reversible post translational modification in all domains of life. While its primary roles appear to reside in metabolic processes, lysine acetylation has also been implicated in regulating pathogenesis in bacteria. By now, several global lysine acetylome analyses have been carried out in various bacteria, but thus far there have been no reports of lysine acetylation taking place in the important human pathogen Vibrio cholerae. In this study, we analyzed the lysine acetylproteome of the human pathogen V. cholerae V52. By applying a combination of immuno-enrichment of acetylated peptides and high resolution mass spectrometry, we identified 3402 acetylation sites on 1240 proteins. Of the acetylated proteins, more than half were acetylated on two or more sites. As reported for other bacteria, we observed that many of the acetylated proteins were involved in metabolic and cellular processes and there was an over-representation of acetylated proteins involved in protein synthesis. Of interest, we demonstrated that many global transcription factors such as CRP, H-NS, IHF, Lrp and RpoN as well as transcription factors AphB, TcpP, PhoB, and ToxR, involved in direct regulation of virulence in V. cholerae were acetylated. In addition VAS effector proteins involved in type VI secretion were acetylated. The overall level of acetylation increased during growth and was higher in stationary phase. In conclusion, this is the first global protein lysine acetylome analysis of V. cholerae and should constitute a valuable resource for in-depth studies of the impact of lysine acetylation in pathogenesis and other cellular processes.
HostingRepository	PRIDE
AnnounceDate	2018-01-02
AnnouncementXML	Submission_2018-01-30_06:29:57.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vaishnavi Ravikumar
SpeciesList	scientific name: Vibrio cholerae V52; NCBI TaxID: 345076;
ModificationList	acetylated residue
Instrument	Q Exactive; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2017-10-26 06:12:06	ID requested
1	2018-01-02 01:48:48	announced
⏵ 2	2018-01-30 06:29:58	announced	Updated publication reference for PubMed record(s): 29376036.

Jers C, Ravikumar V, Lezyk M, Sultan A, Sj, ö, ling Å, Wai SN, Mijakovic I, V52 Reveals Lysine Acetylation of Major Transcriptional Regulators. Front Cell Infect Microbiol, 7():537(2017) [pubmed]

curator keyword: Biological

submitter keyword: Vibrio cholerae, Pathogen, Bacteria, Lysine acetylation, Acetylome, Mass spectrometry, Proteomics

Ivan Mijakovic
contact affiliation	Professor, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kemitorvet 220, DK-2800 Lyngby, Denmark
contact email	ivmi@biosustain.dtu.dk
lab head
Vaishnavi Ravikumar
contact affiliation	Technical University of Denmark
contact email	vairav@biosustain.dtu.dk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD008055
     1. Label: PRIDE project
     2. Name: The global acetylome of the human pathogen Vibrio cholerae V52 reveals lysine acetylation of major transcriptional regulators