PXD008051

PXD008051 is an original dataset announced via ProteomeXchange.

Title	Translational repression of pre-formed cytokine mRNA prevents chronic activation of memory T cells
Description	Memory T cells are critical to protect us from recurring infections. Their instantaneous reactivity to pathogens is empowered by persistent expression of cytokine mRNA. How aberrant protein production of this pre-formed mRNA is prevented in the absence of infection, however, remains unresolved. We show that protein production in memory T cells is blocked through a 3’untranslated region (3’UTR)-mediated process, and that AU-rich elements (AREs) are key herein. Germ-line deletion of AREs leads to chronic IFN- production in bona fide memory T cells. Strikingly, the aberrant protein production does not result from increased mRNA levels and/or half-life. Rather, AREs block the recruitment of cytokine mRNA to ribosomes, a process that is mediated by the ARE-binding protein ZFP36L2. Thus, AREs are crucial elements for translational repression that allow murine and human memory T cells to contain pre-formed cytokine mRNAs, while preventing undesirable protein production in the absence of infection.
HostingRepository	PRIDE
AnnounceDate	2018-05-15
AnnouncementXML	Submission_2019-03-07_05:39:25.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Maartje van den Biggelaar
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion ETD

Revision	Datetime	Status	ChangeLog Entry
0	2017-10-26 04:31:04	ID requested
1	2018-05-15 05:48:31	announced
⏵ 2	2019-03-07 05:39:35	announced	Updated publication reference for PubMed record(s): 29988089.

Salerno F, Engels S, van den Biggelaar M, van Alphen FPJ, Guislain A, Zhao W, Hodge DL, Bell SE, Medema JP, von Lindern M, Turner M, Young HA, Wolkers MC, Translational repression of pre-formed cytokine-encoding mRNA prevents chronic activation of memory T cells. Nat Immunol, 19(8):828-837(2018) [pubmed]

curator keyword: Biological

submitter keyword: memory T cells, Orbitrap Fusion ETD, mouse

Maartje van den Biggelaar
contact affiliation	Department of Plasma Proteins, laboratory of Proteomics, Sanquin Research, The Netherlands
contact email	m.vandenbiggelaar@sanquin.nl
lab head
Maartje van den Biggelaar
contact affiliation	Sanquin Research
contact email	m.vandenbiggelaar@sanquin.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/05/PXD008051

PRIDE project URI

• PRIDE
  1. PXD008051
     1. Label: PRIDE project
     2. Name: Translational repression of pre-formed cytokine mRNA prevents chronic activation of memory T cells