PXD008050

PXD008050 is an original dataset announced via ProteomeXchange.

Title	UVA and to UV-oxidized lipids proteomic regulation of the human keratinocytes
Description	Ultraviolet light is the dominant environmental oxidative skin stressor and a major skin aging factor. We studied which oxidized phospholipid (OxPL) mediators Ultraviolet A (UVA) would generate in primary human keratinocytes (KC). Mass spectrometric analysis of the oxidized phospholipidome of KC immediately or 24h post stress revealed dynamic changes in abundance of 174 oxidized phosphocholine species. Exposure to UVA and to in vitro UVA - oxidized phospholipids both activated, on transcriptome and proteome level, NRF2/antioxidant response signaling and lipid metabolizing enzyme expression, whereas UVA additionally initiated the unfolded protein response (UPR). We identified Nupr1 as an upstream transcriptional regulator of UVA/OxPL mediated gene expression that is itself transcriptionally regulated by reactive lipids, which also aggregate and crosslink recombinant Nupr1 protein. Nupr1 governs the basal and stress regulated expression of cell cycle, redox reactive, autophagy- and lipid metabolizing genes in epidermal keratinocytes, making it a potential key factor in skin ROS responses, -aging and -pathology.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:41:30.071.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fernando J. Sialana
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive; Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2017-10-26 04:27:10	ID requested
1	2019-05-27 04:41:04	announced
⏵ 2	2024-10-22 04:41:38	announced	2024-10-22: Updated project metadata.

Narzt MS, Nagelreiter IM, Oskolkova O, Bochkov VN, Latreille J, Fedorova M, Ni Z, Sialana FJ, Lubec G, Filzwieser M, Laggner M, Bilban M, Mildner M, Tschachler E, Grillari J, Gruber F, A novel role for NUPR1 in the keratinocyte stress response to UV oxidized phospholipids. Redox Biol, 20():467-482(2019) [pubmed]

10.1016/j.redox.2018.11.006;

curator keyword: Biological

submitter keyword: UV, keratinocytes, oxidized lipids,human

Gert Lubec
contact affiliation	University of Vienna
contact email	gert.lubec@lubeclab.com
lab head
Fernando J. Sialana
contact affiliation	Institute of Cancer Research
contact email	fsialana@yahoo.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/05/PXD008050

PRIDE project URI

• PRIDE
  1. PXD008050
     1. Label: PRIDE project
     2. Name: UVA and to UV-oxidized lipids proteomic regulation of the human keratinocytes