PXD008041 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic characterization of the heart and skeletal muscle reveals widespread Arginine ADP-ribosylation by the ectopic ADP-ribosyltransferase ARTC1 |
Description | ADP-ribosylation has thus far been studied on the proteomic level mainly in cell lines and in combination with genotoxic stress. The clostridium-like ADP-ribosyltransferases (i.e. ARTC) are GPI-anchored or secreted proteins that are expressed in a highly tissue specific manner. Transcriptomic data revealed that ARTC1 is expressed in skeletal muscle and heart tissue. Although ARTC1 is highly active in vitro, identification of ARTC1 targets in vivo and subsequent characterization of ARTC1-regulated cellular processes on the proteome level has been challenging and only a few ADP-ribosylated targets are known. Using a new MS-based workflow, we provide the thus far most extensive identification of endogenous ADP-ribosylomes with hundreds of ARTC1-ADP-ribosylated proteins in C2C12 myotubes and in skeletal muscle and heart tissues from wild type and newly generated ARTC1 knockout mice. These proteins are ADP-ribosylated on arginine residues and mainly located on the cell surface or in the extracellular space. They are associated with signal transduction, transmembrane transport and muscle function. Together we provide the first comprehensive systems-level analysis of endogenous ADP-ribosylation in two different muscle tissues revealing a widespread function of ARTC1 and its targets. |
HostingRepository | PRIDE |
AnnounceDate | 2018-08-17 |
AnnouncementXML | Submission_2018-08-20_03:14:52.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD008041 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Mario Leutert |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | Oxidation; Oxidation; Carbamidomethyl |
Instrument | Q Exactive HF; Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-10-25 05:44:48 | ID requested | |
1 | 2018-08-17 01:56:28 | announced | |
⏵ 2 | 2018-08-20 03:14:54 | announced | Updated publication reference for PubMed record(s): 30110646. |
Publication List
Leutert M, Menzel S, Braren R, Rissiek B, Hopp AK, Nowak K, Bisceglie L, Gehrig P, Li H, Zolkiewska A, Koch-Nolte F, Hottiger MO, Proteomic Characterization of the Heart and Skeletal Muscle Reveals Widespread Arginine ADP-Ribosylation by the ARTC1 Ectoenzyme. Cell Rep, 24(7):1916-1929.e5(2018) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: ADP-ribosylation, PTM, ARTC1, Art1, Heart, Muscle, C2C12, HCD, EThcD, |
Contact List
Michael O. Hottiger |
contact affiliation | Department of Molecular Mechanisms of Disease, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland |
contact email | michael.hottiger@dmmd.uzh.ch |
lab head | |
Mario Leutert |
contact affiliation | University of Zurich |
contact email | marioleutert@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD008041
- Label: PRIDE project
- Name: Proteomic characterization of the heart and skeletal muscle reveals widespread Arginine ADP-ribosylation by the ectopic ADP-ribosyltransferase ARTC1