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PXD008040

PXD008040 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSMARCA4 Phosphorilation inhibition
DescriptionCyclin-Dependent Kinase 9 (CDK9) as part of the PTEFb complex promotes transcriptional elongation and high-level gene expression. We now report that, paradoxically, CDK9 is also essential for maintaining gene silencing at heterochromatic loci. Through a live cell screen, we discovered that CDK9 inhibition reactivates epigenetically silenced genes in cancer, leading to restored tumor suppressor gene expression and cell differentiation, along with activation of endogenous retrovirus (ERV) genes. CDK9 inhibition maintains gene silencing directly through phosphorylation of the SWI/SNF protein SMARCA4 and indirectly through HP1α activation. Based on gene activation, we developed the highly selective and 2 potent CDK9 inhibitor MC180295 (IC50 =5.1nM) that has broad anti-cancer activity in-vitro and is effective in in-vivo cancer models. Additionally, CDK9 inhibition synergizes with the immune checkpoint inhibitor α-PD-1 in vivo, making it an excellent target for epigenetic therapy of cancer.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_09:00:44.649.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterCarlos Barrero
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListcarbamoylated residue; monohydroxylated residue; acetylated residue
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-10-25 03:15:34ID requested
12023-06-13 14:36:41announced
22023-11-14 09:00:49announced2023-11-14: Updated project metadata.
Publication List
Zhang H, Pandey S, Travers M, Sun H, Morton G, Madzo J, Chung W, Khowsathit J, Perez-Leal O, Barrero CA, Merali C, Okamoto Y, Sato T, Pan J, Garriga J, Bhanu NV, Simithy J, Patel B, Huang J, Raynal NJ, Garcia BA, Jacobson MA, Kadoch C, Merali S, Zhang Y, Childers W, Abou-Gharbia M, Karanicolas J, Baylin SB, Zahnow CA, Jelinek J, Gra, ñ, a X, Issa JJ, Targeting CDK9 Reactivates Epigenetically Silenced Genes in Cancer. Cell, 175(5):1244-1258.e26(2018) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Phosphoproteomics, Colon Cancer Cells, LC-MS/MS
Contact List
Salim Merali
contact affiliationProfessor of Pharmaceutical Sciences & Moulder Center for Drug Discovery Professor Lewis Katz School of Medicine Member Fox Chase Cancer Institute Director of Proteomics/Metabolomics Temple University School of Pharmacy
contact emailsmerali@temple.edu
lab head
Carlos Barrero
contact affiliationAssistant Professor Pharmacy School Temple University
contact emailcbarrero@temple.edu
dataset submitter
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Dataset FTP location
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