PXD008040
PXD008040 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | SMARCA4 Phosphorilation inhibition |
Description | Cyclin-Dependent Kinase 9 (CDK9) as part of the PTEFb complex promotes transcriptional elongation and high-level gene expression. We now report that, paradoxically, CDK9 is also essential for maintaining gene silencing at heterochromatic loci. Through a live cell screen, we discovered that CDK9 inhibition reactivates epigenetically silenced genes in cancer, leading to restored tumor suppressor gene expression and cell differentiation, along with activation of endogenous retrovirus (ERV) genes. CDK9 inhibition maintains gene silencing directly through phosphorylation of the SWI/SNF protein SMARCA4 and indirectly through HP1α activation. Based on gene activation, we developed the highly selective and 2 potent CDK9 inhibitor MC180295 (IC50 =5.1nM) that has broad anti-cancer activity in-vitro and is effective in in-vivo cancer models. Additionally, CDK9 inhibition synergizes with the immune checkpoint inhibitor α-PD-1 in vivo, making it an excellent target for epigenetic therapy of cancer. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_09:00:44.649.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Carlos Barrero |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | carbamoylated residue; monohydroxylated residue; acetylated residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2017-10-25 03:15:34 | ID requested | |
1 | 2023-06-13 14:36:41 | announced | |
⏵ 2 | 2023-11-14 09:00:49 | announced | 2023-11-14: Updated project metadata. |
Publication List
Zhang H, Pandey S, Travers M, Sun H, Morton G, Madzo J, Chung W, Khowsathit J, Perez-Leal O, Barrero CA, Merali C, Okamoto Y, Sato T, Pan J, Garriga J, Bhanu NV, Simithy J, Patel B, Huang J, Raynal NJ, Garcia BA, Jacobson MA, Kadoch C, Merali S, Zhang Y, Childers W, Abou-Gharbia M, Karanicolas J, Baylin SB, Zahnow CA, Jelinek J, Gra, ñ, a X, Issa JJ, Targeting CDK9 Reactivates Epigenetically Silenced Genes in Cancer. Cell, 175(5):1244-1258.e26(2018) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Phosphoproteomics, Colon Cancer Cells, LC-MS/MS |
Contact List
Salim Merali | |
---|---|
contact affiliation | Professor of Pharmaceutical Sciences & Moulder Center for Drug Discovery Professor Lewis Katz School of Medicine Member Fox Chase Cancer Institute Director of Proteomics/Metabolomics Temple University School of Pharmacy |
contact email | smerali@temple.edu |
lab head | |
Carlos Barrero | |
contact affiliation | Assistant Professor Pharmacy School Temple University |
contact email | cbarrero@temple.edu |
dataset submitter |
Full Dataset Link List
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PRIDE project URI |
Repository Record List
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