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PXD008035

DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2018-06-13
  • AnnouncementXML: Submission_2018-06-13_07:57:38.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: Amanda Assoni
  • Title: Mesenchymal stem cells enhance tumorigenic properties of human glioblastoma cells through independent cell-cell communication mechanisms
  • Description: Mesenchymal stem cells (MSC) display tumor tropism and have been addressed as vehicles for delivery of anti-cancer agents. As cellular components of the tumor microenvironment, MSC also influence tumor progression. However, the contribution of MSC in brain cancer is not well understood since either oncogenic or tumor suppressor effects have been reported for these cells. Here, MSC were found capable of stimulating human Glioblastoma (GBM) cell proliferation through a paracrine effect mediated by TGFB1. Moreover, when in direct cell-cell contact with GBM cells, MSC elicited an increased proliferative and invasive tumor cell behavior under 3D conditions, as well as accelerated tumor development in nude mice, independently of paracrine TGFB1. A secretome profiling of MSC-GBM co-cultures identified 126 differentially expressed proteins and 10 proteins exclusively detected under direct cell-cell contact conditions. Most of these proteins are exosome cargos and are involved in cell motility and tissue development. These results indicate a dynamic interaction between MSC and GBM cells, favoring aggressive tumor cell traits through alternative and independent mechanisms. Overall, these findings indicate that MSC may exert pro-tumorigenic effects when in close contact with tumor cells, which must be carefully considered when employing MSC in targeted cell therapy protocols against cancer
  • SpeciesList: scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
  • ModificationList: iodoacetamide derivatized residue
  • Instrument: Q Exactive

Dataset History

VersionDatetimeStatusChangeLog Entry
02017-10-24 07:31:46ID requested
12018-06-13 07:57:40announced

Publication List

  1. Rodini CO, Gonçalves da Silva PB, Assoni AF, Carvalho VM, Okamoto OK, Mesenchymal stem cells enhance tumorigenic properties of human glioblastoma through independent cell-cell communication mechanisms. Oncotarget, 9(37):24766-24777(2018) [pubmed]

Keyword List

  1. curator keyword: Biological, Biomedical
  2. submitter keyword: Glioblastoma, tumor microenvironment, mesenchymal stem cells, aggressiveness

Contact List

    Oswaldo Keith Okamoto
    • contact affiliation: Universidade de São Paulo
    • contact email: keith.okamoto@usp.br
    • lab head:
    Amanda Assoni
    • contact affiliation: USP
    • contact email: amanda_assoni@hotmail.com
    • dataset submitter:

Full Dataset Link List

  1. Dataset FTP location
  2. PRIDE project URI
Repository Record List
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