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PXD008034

DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2018-09-13
  • AnnouncementXML: Submission_2018-09-13_11:09:37.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: Gustavo De Souza
  • Title: Proteome analysis of Escherichia coli ptsN-null strain under different nitrogen regimes
  • Description: The carbohydrate-uptake phosphorelay PTS system plays a key role in metabolic regulation in Bacteria controlling the utilization of secondary carbon sources. Some Bacteria, such as Escherichia coli, encode a paralogue system named PTSNtr (nitrogen related PTS). PTSNtr is composed of EINtr (ptsP), NPr (ptsO), and EIIANtr (ptsN). These proteins act as a phosphorely system transfering phosphoryl from phosphoenolpyruvate to EINtr, NPr and them to EIIANtr. PTSNtr is not involved in carbohydrate uptake and it may be dedicated to play regulatory functions. The phosphorylation state of the EINtr is regulated by allosteric binding of glutamine and 2-oxoglutarate, metabolites whose intracellular levels reflect the nitrogen levels. Despite the denomination and nitrogen-sensory properties no major effect of PTSNtr on nitrogen regulation has been described in E. coli. Here we show that an E. coli ∆ptsN has impaired growth in minimal medium. Proteome analysis of the ∆ptsN strain under different nitrogen regimes revealed that ptsN is not involved in the regulation of the canonical nitrogen starvation response. The proteomic data support that ptsN is required for proper balance between RpoS and sigma 70 activities such that, in the absence of ptsN, RpoS-depended genes are preferentially expressed.
  • SpeciesList: scientific name: Escherichia coli; NCBI TaxID: 562;
  • ModificationList: monohydroxylated residue; iodoacetamide derivatized residue
  • Instrument: Q Exactive

Dataset History

VersionDatetimeStatusChangeLog Entry
02017-10-24 07:18:23ID requested
12018-09-13 11:09:38announced

Publication List

  1. Gravina F, Sanchuki HS, Rodrigues TE, Gerhardt ECM, Pedrosa FO, Souza EM, Valdameri G, de Souza GA, Huergo LF, Proteome analysis of an Escherichia coli ptsN-null strain under different nitrogen regimes. J Proteomics, 174():28-35(2018) [pubmed]

Keyword List

  1. curator keyword: Biological
  2. submitter keyword: nitrogen, Ntr, PTS, PII, Proteome

Contact List

    Luciano Huergo
    • contact affiliation: Departamento de Bioquímica e Biologia Molecular, UFPR, Curitiba, PR, Brazil
    • contact email: huergo@ufpr.br
    • lab head:
    Gustavo De Souza
    • contact affiliation: Brain Institute, Federal University of Rio Grande do Norte
    • contact email: gadsouza@gmail.com
    • dataset submitter:

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