PXD008033

PXD008033 is an original dataset announced via ProteomeXchange.

Title	Chromatin protein profiling during the mammalian cell cycle reveals the extent of mitotic preservation of the regulatory landscape
Description	While regulation of transcription, replication and cell division relies on dynamic protein binding to DNA and chromatin, it is unclear which regulatory components remain bound to compacted mitotic chromosomes. To comprehensively quantify the chromatin-associated proteome in different phases of the cell cycle, we utilized the buoyant density of DNA-protein complexes after crosslinking. This revealed variable associations for thousands of proteins to DNA including their frequent and variable phosphorylation. While epigenetic modifiers that promote transcription are lost from mitotic chromatin, repressive modifiers generally remain associated. Interestingly, while proteins involved in transcriptional elongation are evicted, most identified transcription factors are retained on mitotic chromatin to varying degrees, including core promoter binding proteins. This predicts conservation of the regulatory landscape on mitotic chromosomes, which genome-wide measurements of chromatin accessibility confirm. This work establishes a novel approach to study chromatin, provides a comprehensive catalogue of chromatin changes during the cell cycle, and reveals the degree with which the genomic regulatory landscape is maintained through mitosis.
HostingRepository	PRIDE
AnnounceDate	2018-09-06
AnnouncementXML	Submission_2019-04-03_04:31:09.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Paul Ginno
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; phosphorylated residue; N6: N6; acetylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2017-10-24 07:16:35	ID requested
1	2018-09-05 23:50:01	announced
⏵ 2	2019-04-03 04:31:11	announced	Updated publication reference for PubMed record(s): 30279501.

Ginno PA, Burger L, Seebacher J, Iesmantavicius V, Sch, ü, beler D, Cell cycle-resolved chromatin proteomics reveals the extent of mitotic preservation of the genomic regulatory landscape. Nat Commun, 9(1):4048(2018) [pubmed]

curator keyword: Biological

submitter keyword: Chromatin, Cell Cycle, Mitotic Bookmarking

Dirk Schübeler
contact affiliation	Friedrich Miescher Institute for Biomedical Research Epigenetics Department Lab 1.22
contact email	dirk.schubeler@fmi.ch
lab head
Paul Ginno
contact affiliation	FMI
contact email	paul.ginno@fmi.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD008033
     1. Label: PRIDE project
     2. Name: Chromatin protein profiling during the mammalian cell cycle reveals the extent of mitotic preservation of the regulatory landscape