⮝ Full datasets listing

PXD008027

PXD008027 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleChlorosis as developmental program in cyanobacteria: The proteomic fundament for surviving and awakening
DescriptionCyanobacteria that do not fix nitrogen adapt to prolonged periods of nitrogen deprivation in a chlorotic state where cell growth and metabolism is arrested. Upon nutrient availability, the cells return back to vegetative growth within 2-3 days. This resuscitation process is highly orchestrated and relies on the stepwise re-installation and activation of essential cellular structures and functions. We investigate transition into chlorosis and return to vegetative life as a simple model of a cellular developmental process that represents a fundamental survival strategy in biology. In the present study, we describe the process through quantitative proteomics and phosphoproteomics analysis of long-term nitrogen-starved Synechocystis sp. PCC 6803 cells during different time points of resuscitation. Intriguingly, substantial O phosphorylation of proteins involved in photosynthesis was detected in the chlorotic state, including hyperphosphorylation of the remaining phycobiliproteins. We provide evidence that hyperphosphorylation of the terminal rod linker CpcD increases the lifespan of phycobiliproteins during chlorosis.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:41:23.137.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterPhilipp Spaet
SpeciesList scientific name: Synechocystis sp. PCC 6803; NCBI TaxID: 1148;
ModificationListphosphorylated residue
InstrumentQ Exactive; LTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-10-24 02:24:39ID requested
12018-06-28 08:19:20announced
22024-10-22 04:41:31announced2024-10-22: Updated project metadata.
Publication List
10.1074/mcp.ra118.000699;
Sp, ä, t P, Klotz A, Rexroth S, Ma, č, ek B, Forchhammer K, Chlorosis as a Developmental Program in Cyanobacteria: The Proteomic Fundament for Survival and Awakening. Mol Cell Proteomics, 17(9):1650-1669(2018) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Synechocystis sp. PCC 6803 phosphoproteome dynamics during resuscitation form nitrogen-chlorosis
Contact List
Boris Macek
contact affiliationProf. Dr. Boris Macek Chair, Quantitative Proteomics Director, Proteome Center Tuebingen Interfaculty Institute for Cell Biology University of Tuebingen Auf der Morgenstelle 15 72076 Tuebingen Germany Phone: +49/(0)7071/29-70556 Fax: +49/(0)7071/29-5779
contact emailboris.macek@uni-tuebingen.de
lab head
Philipp Spaet
contact affiliationUniversity Tuebingen Department of Quantitative Proteomics
contact emailphilipp.spaet@uni-tuebingen.de
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008027
PRIDE project URI
Repository Record List
[ + ]