PXD008027

PXD008027 is an original dataset announced via ProteomeXchange.

Title	Chlorosis as developmental program in cyanobacteria: The proteomic fundament for surviving and awakening
Description	Cyanobacteria that do not fix nitrogen adapt to prolonged periods of nitrogen deprivation in a chlorotic state where cell growth and metabolism is arrested. Upon nutrient availability, the cells return back to vegetative growth within 2-3 days. This resuscitation process is highly orchestrated and relies on the stepwise re-installation and activation of essential cellular structures and functions. We investigate transition into chlorosis and return to vegetative life as a simple model of a cellular developmental process that represents a fundamental survival strategy in biology. In the present study, we describe the process through quantitative proteomics and phosphoproteomics analysis of long-term nitrogen-starved Synechocystis sp. PCC 6803 cells during different time points of resuscitation. Intriguingly, substantial O phosphorylation of proteins involved in photosynthesis was detected in the chlorotic state, including hyperphosphorylation of the remaining phycobiliproteins. We provide evidence that hyperphosphorylation of the terminal rod linker CpcD increases the lifespan of phycobiliproteins during chlorosis.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:41:23.137.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Philipp Spaet
SpeciesList	scientific name: Synechocystis sp. PCC 6803; NCBI TaxID: 1148;
ModificationList	phosphorylated residue
Instrument	Q Exactive; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2017-10-24 02:24:39	ID requested
1	2018-06-28 08:19:20	announced
⏵ 2	2024-10-22 04:41:31	announced	2024-10-22: Updated project metadata.

10.1074/mcp.ra118.000699;

Sp, ä, t P, Klotz A, Rexroth S, Ma, č, ek B, Forchhammer K, Chlorosis as a Developmental Program in Cyanobacteria: The Proteomic Fundament for Survival and Awakening. Mol Cell Proteomics, 17(9):1650-1669(2018) [pubmed]

curator keyword: Biological

submitter keyword: Synechocystis sp. PCC 6803 phosphoproteome dynamics during resuscitation form nitrogen-chlorosis

Boris Macek
contact affiliation	Prof. Dr. Boris Macek Chair, Quantitative Proteomics Director, Proteome Center Tuebingen Interfaculty Institute for Cell Biology University of Tuebingen Auf der Morgenstelle 15 72076 Tuebingen Germany Phone: +49/(0)7071/29-70556 Fax: +49/(0)7071/29-5779
contact email	boris.macek@uni-tuebingen.de
lab head
Philipp Spaet
contact affiliation	University Tuebingen Department of Quantitative Proteomics
contact email	philipp.spaet@uni-tuebingen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008027

PRIDE project URI

• PRIDE
  1. PXD008027
     1. Label: PRIDE project
     2. Name: Chlorosis as developmental program in cyanobacteria: The proteomic fundament for surviving and awakening