PXD008027 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Chlorosis as developmental program in cyanobacteria: The proteomic fundament for surviving and awakening |
Description | Cyanobacteria that do not fix nitrogen adapt to prolonged periods of nitrogen deprivation in a chlorotic state where cell growth and metabolism is arrested. Upon nutrient availability, the cells return back to vegetative growth within 2-3 days. This resuscitation process is highly orchestrated and relies on the stepwise re-installation and activation of essential cellular structures and functions. We investigate transition into chlorosis and return to vegetative life as a simple model of a cellular developmental process that represents a fundamental survival strategy in biology. In the present study, we describe the process through quantitative proteomics and phosphoproteomics analysis of long-term nitrogen-starved Synechocystis sp. PCC 6803 cells during different time points of resuscitation. Intriguingly, substantial O phosphorylation of proteins involved in photosynthesis was detected in the chlorotic state, including hyperphosphorylation of the remaining phycobiliproteins. We provide evidence that hyperphosphorylation of the terminal rod linker CpcD increases the lifespan of phycobiliproteins during chlorosis. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:41:23.137.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Philipp Spaet |
SpeciesList | scientific name: Synechocystis sp. PCC 6803; NCBI TaxID: 1148; |
ModificationList | phosphorylated residue |
Instrument | Q Exactive; LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-10-24 02:24:39 | ID requested | |
1 | 2018-06-28 08:19:20 | announced | |
⏵ 2 | 2024-10-22 04:41:31 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1074/mcp.ra118.000699; |
Sp, ä, t P, Klotz A, Rexroth S, Ma, č, ek B, Forchhammer K, Chlorosis as a Developmental Program in Cyanobacteria: The Proteomic Fundament for Survival and Awakening. Mol Cell Proteomics, 17(9):1650-1669(2018) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Synechocystis sp. PCC 6803 phosphoproteome dynamics during resuscitation form nitrogen-chlorosis |
Contact List
Boris Macek |
contact affiliation | Prof. Dr. Boris Macek Chair, Quantitative Proteomics Director, Proteome Center Tuebingen Interfaculty Institute for Cell Biology University of Tuebingen Auf der Morgenstelle 15 72076 Tuebingen Germany Phone: +49/(0)7071/29-70556 Fax: +49/(0)7071/29-5779 |
contact email | boris.macek@uni-tuebingen.de |
lab head | |
Philipp Spaet |
contact affiliation | University Tuebingen Department of Quantitative Proteomics |
contact email | philipp.spaet@uni-tuebingen.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD008027
- Label: PRIDE project
- Name: Chlorosis as developmental program in cyanobacteria: The proteomic fundament for surviving and awakening