PXD008019

PXD008019 is an original dataset announced via ProteomeXchange.

Title	First dataset - bacterial proteome of carbapanemase-producing Escherichia coli and other Enterobacteriaceae under antibiotic pressure
Description	Antibiotic resistance associated with the expression of the clinically significant carbapenemases, IMP, KPC, and NDM and OXA-48 in Enterobacteriaceae is emerging as a worldwide calamity to health care. In Australia, IMP-producing Enterobacteriaceae is the most prevalent carbapenemase-producing Enterobacteriaceae (CPE). Genomic characteristics of such carbapenemase-producing Enterobacteriaceae (CPE) are well described, but the corresponding proteome is poorly characterised. We have thus developed a method to analyse dynamic changes in the proteome of CPE under antibiotic pressure. Specifically, we have investigated the effect of meropenem at sub-lethal concentrations to develop a better understanding of how antibiotic pressure leads to resistance. Escherichia coli, producing either NDM, IMP or KPC type carbapenemase were included in this study, and their proteomes were analysed in growth conditions with or without meropenem.
HostingRepository	PRIDE
AnnounceDate	2018-07-11
AnnouncementXML	Submission_2018-07-11_09:29:25.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hanna Sidjabat
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2017-10-23 02:35:04	ID requested
⏵ 1	2018-07-11 09:29:26	announced

Sidjabat HE, Gien J, Kvaskoff D, Ashman K, Vaswani K, Reed S, McGeary RP, Paterson DL, Bordin A, Schenk G, The use of SWATH to analyse the dynamic changes of bacterial proteome of carbapanemase-producing Escherichia coli under antibiotic pressure. Sci Rep, 8(1):3871(2018) [pubmed]

curator keyword: Biomedical

submitter keyword: Antibiotic resistance, proteomics, SWATH analysis, carbapenemases, IMP, NDM, KPC

Hanna Sidjabat
contact affiliation	The University of Queensland, Centre for Clinical Research, Faculty of Medicine.
contact email	h.sidjabat@uq.edu.au
lab head
Hanna Sidjabat
contact affiliation	The University of Queensland
contact email	h.sidjabat@uq.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/07/PXD008019

PRIDE project URI

• PRIDE
  1. PXD008019
     1. Label: PRIDE project
     2. Name: First dataset - bacterial proteome of carbapanemase-producing Escherichia coli and other Enterobacteriaceae under antibiotic pressure