PXD008015

PXD008015 is an original dataset announced via ProteomeXchange.

Title	Dual-compartment shotgun proteomics unravel thermal stress responses in reef-calcifiers and their photosymbionts
Description	The proliferation of coral reef organisms, such as the key ecological engineers and carbonate producers’ large benthic foraminifera (LBF), greatly relies on the mutual association with photosymbiotic algae. With respect to global change impacts such as ocean warming, evaluating stress and acclimatization responses is highly important, but probing proteins and partitioning host and symbionts are major obstacles for studying LBF. We therefore applied a label-free quantitative proteomics approach to LBF holobionts in order to detect changes in the relative abundances of proteins in response to different thermal-stress scenarios. Subsequent identification allowed for the partial assignment of 1,618 proteins to either foraminiferal host or endosymbiotic diatoms. While single and episodic thermal-stress events only induced minor metabolic impacts, chronic thermal stress diminished photosynthesis-related proteins among the symbionts. Stress response and proteolysis-related proteins increased in abundance, illustrating the extent of damage. In contrast to the symbionts, host homeostasis was maintained through extensive repair mechanisms and high protein turnover. Metabolic pathways were adjusted to the symbiont loss by using alternative energy resources. However, linking proteome to physiological data revealed that resource redistribution impairs growth and certainly other functions that could disturb carbonate production. We show how compartment partitioning can give insights into interactions and stress response mechanisms in photosymbiotic reef-calcifiers.
HostingRepository	PRIDE
AnnounceDate	2018-02-27
AnnouncementXML	Submission_2018-02-27_05:49:00.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bernhard Blank-Landeshammer
SpeciesList	scientific name: Bacillariophyta; NCBI TaxID: 2836; scientific name: Amphistegina; NCBI TaxID: 1659668;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-10-23 02:05:47	ID requested
⏵ 1	2018-02-27 05:49:01	announced

Stuhr M, Blank-Landeshammer B, Reymond CE, Kollipara L, Sickmann A, Kucera M, Westphal H, Disentangling thermal stress responses in a reef-calcifier and its photosymbionts by shotgun proteomics. Sci Rep, 8(1):3524(2018) [pubmed]

curator keyword: Biological

submitter keyword: LC-MSMS, large benthic foraminifera, thermal stress, label-free quantitation

Albert Sickmann
contact affiliation	Leibniz-Institut für Analytische Wissenschaften – ISAS – e.V., Dortmund, Germany
contact email	sickmann@isas.de
lab head
Bernhard Blank-Landeshammer
contact affiliation	Leibniz-Institut für Analytische Wissenschaften – ISAS – e.V., Dortmund
contact email	bernhard.blank@isas.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/02/PXD008015

PRIDE project URI

• PRIDE
  1. PXD008015
     1. Label: PRIDE project
     2. Name: Dual-compartment shotgun proteomics unravel thermal stress responses in reef-calcifiers and their photosymbionts