PXD008012
PXD008012 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | In vivo phosphoproteomics reveals kinase activity profiles that predict treatment outcome in triple-negative breast cancer |
Description | Triple-negative breast cancer (TNBC) lacks prognostic and predictive markers. We used high-throughput phosphoproteomics to build a functional TNBC taxonomy. A cluster of 159 phosphosites was upregulated in relapsed cases of a training set (n=34 patients). Eleven hyperactive kinases accounted for this phosphoprofile. A massspectrometry-to-immunohistochemistry translation step that assessed 113 TNBC validation specimens revealed that 6/12 kinases (PRKCE, KIT, PNKP, ERK, CDK6, and P70S6K) preserved independent prognostic value. The kinases split the validation set into two patterns: one (0/6 hyperactive kinases; 29% of the patients) associated with a 93.5% cure rate. The other (≥1 hyperactive kinase; 37 subpatterns) was associated with a 9.5-fold higher relapse risk. Each kinase pattern agglutinated different mutational patterns. Drug regimens designed based on these six kinases showed synergistic activity in vitro and in vivo. Our results elucidated novel phosphosites/kinases that are implicated in TNBC and provide a ready-to-use, actionable target-based clinical classification system for TNBC |
HostingRepository | PRIDE |
AnnounceDate | 2018-07-23 |
AnnouncementXML | Submission_2019-03-07_05:40:01.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Javier Muñoz |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2017-10-23 02:04:44 | ID requested | |
1 | 2018-07-19 03:16:40 | announced | |
2 | 2018-07-24 05:27:35 | announced | Updated contact info |
⏵ 3 | 2019-03-07 05:40:03 | announced | Updated publication reference for PubMed record(s): 30158526. |
Publication List
Zagorac I, Fernandez-Gaitero S, Penning R, Post H, Bueno MJ, Mouron S, Manso L, Morente MM, Alonso S, Serra V, Mu, ñ, oz J, G, ó, mez-L, ó, pez G, Lopez-Acosta JF, Jimenez-Renard V, Gris-Oliver A, Al-Shahrour F, Pi, ñ, eiro-Ya, ñ, ez E, Montoya-Suarez JL, Apala JV, Moreno-Torres A, Colomer R, Dopazo A, Heck AJR, Altelaar M, Quintela-Fandino M, In vivo phosphoproteomics reveals kinase activity profiles that predict treatment outcome in triple-negative breast cancer. Nat Commun, 9(1):3501(2018) [pubmed] |
Keyword List
ProteomeXchange project tag: PRIME-XS Project |
curator keyword: Biomedical |
submitter keyword: triple negative breast cancer, phophoproteomics, kinases |
Contact List
Miguel Quintela-Fandino | |
---|---|
contact affiliation | Breast Cancer Clinical Research Unit, CNIO, Spanish National Cancer Research Centre, Spain |
contact email | mquintela@cnio.es |
lab head | |
Javier Muñoz | |
contact affiliation | Breast Cancer Clinical Research Unit, CNIO, Spanish National Cancer Research Centre, Spain |
contact email | jmunozpe@cnio.es |
dataset submitter |
Full Dataset Link List
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PRIDE project URI |
Repository Record List
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