PXD008008

PXD008008 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Total proteome of germinative and dormant axillary buds of the sugarcane cultivar RB867515
Description	Sugarcane is the raw material for the production of ethanol and sugar, and its by-products are used in cogeneration of energy in addition to various industrial processes. The cane field establishment is dependent of the axillary buds sprouting. The present study aimed to catalog the total proteome of germinative axillary bud and dormant axillary buds, and to identify important budding proteins, which are differentially expressed in these types of axillary buds. The proteome was obtained by extraction of total proteins using the TCA acetone method adapted for axillary buds. The experiment was carried out in biological triplicate. Protein samples were submitted to denaturing polyacrylamide gel electrophoresis (SDS-PAGE) and stained with comassie blue. Each lane of the polyacrylamide gel was fractionated (11 slice) and treated for purification and isolation of the polypeptides. Tryptic digestion was performed on each fragment with subsequent UPLC separation (Nano Acquity). The polypeptides were analyzed on Waters® Micromass® ESI-Q-Tof micro ™ mass spectrometer. Protein samples from the germinative and dormant axillary buds showed distinct bands patterns in the SDS-PAGE gel. Mass spectrometry data were analyzed by MASCOT tool (http://www.matrixscience.com).
HostingRepository	jPOST
AnnounceDate	2019-08-04
AnnouncementXML	Submission_2022-09-18_03:42:03.249.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rone Charles Maranho
SpeciesList	scientific name: Saccharum hybrid cultivar RB867515; NCBI TaxID: 1890409;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide
Instrument	Q-Tof micro

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2017-10-19 19:51:20	ID requested
1	2019-08-04 07:32:21	announced
2	2021-02-04 17:30:16	announced	2021-02-04: Updated FTP location.
⏵ 3	2022-09-18 03:42:03	announced	2022-09-18: Updated FTP location.

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: sprouting, budding, Saccharum spp.

submitter keyword: sprouting, budding, Saccharum spp.

Contact List

Mariana Mancini Benez
lab head
Rone Charles Maranho
contact affiliation	State University of Maringá
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000335/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000335/