Updated FTP location. We immobilized SH2 superbinder onto a monolithic capillary column to construct a microreactor to enrich pTyr peptides from minute amount of samples. To evaluate the feasibility of this strategy, we used protein standards, phosphopeptide samples purified from pervanadate-treated HeLa cell digests as well as a protein complex sample. The results showed that this microreactor enabled highly specific enrichment of phosphotyrosine peptides.