PXD007977

PXD007977 is an original dataset announced via ProteomeXchange.

Title	Imipramine affects the expression of proteins involved in oxidative stress and immunity in rat primary glial cell culture
Description	Glial cells (microglia and astrocytes) have recently became appreciated as an important target for antidepressant drugs. Here we report on the results of comprehensive proteomic analysis of the alteration in protein profile of rat primary mixed glial culture exposed to imipramine. Two-dimensional differential in gel electrophoresis method allowed to identify 62 proteins regulated by imipramine hydrochloride. Functional analysis revealed the impact of imipramine on the level of proteins involved in oxidative stress. Imipramine upregulated proteins related to glycolysis but downregulated many mitochondrial proteins also enzymes of oxidative phosphorylation. Moreover, imipramine influenced the proteins engaged in phagocytosis and cell migration. Alteration in the level of large number of structural and plasma membrane associated proteins evidenced a widespread cytoskeleton and membrane rearrangement. Imipramine triggered decrease of mitochondrial membrane potential, impairment of protein synthesis and downregulation of chaperon proteins, what could be related to increased apoptosis. Many imipramine regulated proteins, among them chaperons, cathepsins and annexins are evidenced to be engaged in immunity response. Overall these experimental findings suggest that in response to imipramine, glial cells (mainly microglia) undergo a transition toward more quiescent, metabolically less demanding phenotype.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:41:30.127.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD007977
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Urszula Jankowska
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	phosphorylated residue; deaminated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	micrOTOF-Q II

Revision	Datetime	Status	ChangeLog Entry
0	2017-10-16 08:44:09	ID requested
1	2018-05-17 06:50:43	announced
⏵ 2	2024-10-22 04:41:31	announced	2024-10-22: Updated project metadata.

10.1016/j.jneuroim.2018.04.008;

10.6019/PXD007977;

Kedracka-Krok S, Swiderska B, Bielecka-Wajdman AM, Prus G, Skupien-Rabian B, Jankowska U, Obuchowicz E, Impact of imipramine on proteome of rat primary glial cells. J Neuroimmunol, 320():25-37(2018) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: imipramine

mixed glial cell culture

microglia

proteome

2D-DIGE

Sylwia Kędracka-Krok
contact affiliation	1. Department of Physical Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland 2. Proteomics and Mass Spectrometry Laboratory, Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland
contact email	sylwia.kedracka-krok@uj.edu.pl
lab head
Urszula Jankowska
contact affiliation	Proteomics and Mass Spectrometry Core Facility, Malopolska Centre of Biotechnology, Jagiellonian University
contact email	urszula.jankowska@uj.edu.pl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/05/PXD007977

PRIDE project URI

• PRIDE
  1. PXD007977
     1. Label: PRIDE project
     2. Name: Imipramine affects the expression of proteins involved in oxidative stress and immunity in rat primary glial cell culture