PXD007976

PXD007976 is an original dataset announced via ProteomeXchange.

Title	Landscape of nuclear transport receptor cargo specificity
Description	Nuclear transport receptors (NTRs) recognize localization signals of cargos to facilitate their passage across the central channel of nuclear pore complexes (NPCs). About 30 different NTRs constitute different transport pathways in humans and bind to a multitude of different cargos. The exact cargo spectrum of the majority of NTRs, their specificity and even the extent to which active nucleocytoplasmic transport contributes to protein localization remains largely unknown because of the transient nature of these interactions and the wide dynamic range of cargo concentrations. To systematically map cargo-NTR relationships in situ, we used proximity ligation coupled to mass spectrometry (BioID). We systematically fused the engineered biotin ligase BirA* to 16 NTRs. We estimate that at least one third of the human proteome is subject to active nuclear transport. We quantified the specificity and redundancy in cargo-NTR interactions, and identified transport pathways for various protein complexes and transcription factors. We extended the BioID method by the direct identification of biotinylation sites. This approach enabled us to identify interaction interfaces and to discriminate direct versus piggyback transport mechanisms.
HostingRepository	PRIDE
AnnounceDate	2017-11-14
AnnouncementXML	Submission_2018-01-02_02:44:44.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marie-Therese Mackmull
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-10-16 07:52:43	ID requested
1	2017-11-14 03:36:37	announced
⏵ 2	2018-01-02 02:44:45	announced	Updated publication reference for PubMed record(s): 29254951.

Mackmull MT, Klaus B, Heinze I, Chokkalingam M, Beyer A, Russell RB, Ori A, Beck M, specificity. Mol Syst Biol, 13(12):962(2017) [pubmed]

curator keyword: Biological

submitter keyword: Interaction network, nuclear pore complex, protein transport, proteomics, proximity ligation

Martin Beck
contact affiliation	Structural and Computational Biology Unit, European Molecular Biology Laboratory (EMBL), Meyerhofstr. 1, 69117 Heidelberg, Germany
contact email	martin.beck@embl.de
lab head
Marie-Therese Mackmull
contact affiliation	EMBL
contact email	marie.mackmull@embl.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD007976
     1. Label: PRIDE project
     2. Name: Landscape of nuclear transport receptor cargo specificity