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DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2017-11-14
  • AnnouncementXML: Submission_2018-01-02_02:44:44.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: Marie-Therese Mackmull
  • Title: Landscape of nuclear transport receptor cargo specificity
  • Description: Nuclear transport receptors (NTRs) recognize localization signals of cargos to facilitate their passage across the central channel of nuclear pore complexes (NPCs). About 30 different NTRs constitute different transport pathways in humans and bind to a multitude of different cargos. The exact cargo spectrum of the majority of NTRs, their specificity and even the extent to which active nucleocytoplasmic transport contributes to protein localization remains largely unknown because of the transient nature of these interactions and the wide dynamic range of cargo concentrations. To systematically map cargo-NTR relationships in situ, we used proximity ligation coupled to mass spectrometry (BioID). We systematically fused the engineered biotin ligase BirA* to 16 NTRs. We estimate that at least one third of the human proteome is subject to active nuclear transport. We quantified the specificity and redundancy in cargo-NTR interactions, and identified transport pathways for various protein complexes and transcription factors. We extended the BioID method by the direct identification of biotinylation sites. This approach enabled us to identify interaction interfaces and to discriminate direct versus piggyback transport mechanisms.
  • SpeciesList: scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
  • ModificationList: biotinylated residue
  • Instrument: LTQ Orbitrap Velos

Dataset History

VersionDatetimeStatusChangeLog Entry
02017-10-16 07:52:43ID requested
12017-11-14 03:36:37announced
22018-01-02 02:44:45announcedUpdated publication reference for PubMed record(s): 29254951.

Publication List

  1. Mackmull MT, Klaus B, Heinze I, Chokkalingam M, Beyer A, Russell RB, Ori A, Beck M, Landscape of nuclear transport receptor cargo specificity. Mol Syst Biol, 13(12):962(2017) [pubmed]

Keyword List

  1. curator keyword: Biological
  2. submitter keyword: Interaction network, nuclear pore complex, protein transport, proteomics, proximity ligation

Contact List

    Martin Beck
    • contact affiliation: Structural and Computational Biology Unit, European Molecular Biology Laboratory (EMBL), Meyerhofstr. 1, 69117 Heidelberg, Germany
    • contact email: martin.beck@embl.de
    • lab head:
    Marie-Therese Mackmull
    • contact affiliation: EMBL
    • contact email: marie.mackmull@embl.de
    • dataset submitter:

Full Dataset Link List

  1. Dataset FTP location
  2. PRIDE project URI
Repository Record List
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