PXD007963

PXD007963 is an original dataset announced via ProteomeXchange.

Title	Synechococcus sp. (PCC 7002) analysis by LC-MS/MS
Description	Cyanobacteria fix atmospheric CO2 to biomass and through metabolic engineering can also act as photosynthetic cell factories for sustainable productions of fuels and chemicals. The Calvin cycle is the primary pathway for CO2 fixation in cyanobacteria, algae and C3 plants, and several studies have shown that overexpression of a cyanobacterial Calvin cycle enzyme, bifunctional sedoheptulose-1,7-bisphosphatase/fructose-1,6-bisphosphatase (hereafter BiBPase), enhances CO2 fixation in both plants and algae, although its impact on cyanobacteria has not yet been rigorously studied. Here, we show that overexpression of BiBPase enhanced growth, cell size, and photosynthetic O2 evolution of the cyanobacterium Synechococcus sp. PCC 7002 in an environment with elevated CO2 concentration. Biochemical analysis, immunodetection, and proteomic analysis revealed that overexpression of BiBPase considerably elevated the cellular activities of two rate-limiting enzymes in the Calvin cycle, namely ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) and aldolase, while it repressed several enzymes involved in the respiratory carbon metabolism (e.g. glycolysis and the oxidative pentose phosphate pathway) including glucose-6-phosphate dehydrogenase. Concomitantly, the content of glycogen was significantly reduced while the extracellular carbohydrate content increased. These results indicate that overexpression of BiBPase leads to global reprogramming of carbon metabolism in Synechococcus sp. PCC 7002, promoting photosynthetic carbon fixation and repressing the respiratory carbon catabolism, while altering carbohydrate partitioning.
HostingRepository	PRIDE
AnnounceDate	2019-01-17
AnnouncementXML	Submission_2019-01-17_00:49:05.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD007963
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Joshua Heazlewood
SpeciesList	scientific name: Synechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) (Agmenellum quadruplicatum); NCBI TaxID: 32049;
ModificationList	Deamidated; Oxidation; Acetyl; Carbamidomethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-10-13 03:36:20	ID requested
1	2019-01-17 00:38:27	announced
⏵ 2	2019-01-17 00:49:06	announced	Updated publication reference for PubMed record(s): 29510212.

De Porcellinis AJ, N, ø, rgaard H, Brey LMF, Erstad SM, Jones PR, Heazlewood JL, Sakuragi Y, Overexpression of bifunctional fructose-1,6-bisphosphatase/sedoheptulose-1,7-bisphosphatase leads to enhanced photosynthesis and global reprogramming of carbon metabolism in Synechococcus sp. PCC 7002. Metab Eng, 47():170-183(2018) [pubmed]

curator keyword: Biological

submitter keyword: Synechococcus, cyanobacteria, RUBISCO, CO2 fixation, Calvin cycle

Yumiko Sakuragi
contact affiliation	Department of Plant and Environmental Sciences, University of Copenhagen
contact email	ysa@plen.ku.dk
lab head
Joshua Heazlewood
contact affiliation	University of Melbourne
contact email	botjlh@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD007963
     1. Label: PRIDE project
     2. Name: Synechococcus sp. (PCC 7002) analysis by LC-MS/MS