PXD007960

PXD007960 is an original dataset announced via ProteomeXchange.

Title	RNA-binding activity of TRIM25 is mediated by its PRY/SPRY domain and is required for ubiquitination
Description	TRIM25 is a novel RNA-binding protein and a member of the Tripartite Motif (TRIM) family of E3 ubiquitin ligases, which plays a pivotal role in the innate immune response. Almost nothing is known about its RNA-related roles in cell biology. Furthermore, its RNA-binding domain has not been characterized. Here, we reveal that RNA-binding activity of TRIM25 is mediated by its PRY/SPRY domain, which we postulate to be a novel RNA-binding domain. Using CLIP-seq and SILAC-based co-immunoprecipitation assays, we uncover TRIM25’s endogenous RNA targets and protein binding partners. Finally, we show that the RNAbinding activity of TRIM25 is important for its ubiquitin ligase function. These results reveal new insights into the molecular roles and characteristics of RNA-binding E3 ubiquitin ligases and demonstrate that RNA could be an essential factor for their biological functions.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:41:38.246.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gracjan Michlewski
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	ubiquitination signature dipeptidyl lysine
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-10-13 01:34:16	ID requested
1	2017-10-18 23:59:01	announced
2	2019-03-07 05:41:39	announced	Updated publication reference for PubMed record(s): 29117863.
⏵ 3	2024-10-22 04:41:41	announced	2024-10-22: Updated project metadata.

10.1186/s12915-017-0444-9;

Choudhury NR, Heikel G, Trubitsyna M, Kubik P, Nowak JS, Webb S, Granneman S, Spanos C, Rappsilber J, Castello A, Michlewski G, RNA-binding activity of TRIM25 is mediated by its PRY/SPRY domain and is required for ubiquitination. BMC Biol, 15(1):105(2017) [pubmed]

curator keyword: Biological

submitter keyword: Human, HeLa, SILAC, ubiquitination, RNA

Dr. Gracjan Michlewski
contact affiliation	WTCCB, University of Edinburgh
contact email	Gracjan.Michlewski@ed.ac.uk
lab head
Gracjan Michlewski
contact affiliation	University of Edinburgh
contact email	Gracjan.Michlewski@ed.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD007960
     1. Label: PRIDE project
     2. Name: RNA-binding activity of TRIM25 is mediated by its PRY/SPRY domain and is required for ubiquitination