PXD007958

PXD007958 is an original dataset announced via ProteomeXchange.

Title	Populus metacaspases
Description	Metacaspases (MCs) are cysteine proteases that are implicated in programmed cell death of plants. AtMC9 (Arabidopsis thaliana Metacaspase9) is a member of the Arabidopsis MC family that controls the rapid autolysis of the xylem vessel elements, but its downstream targets in the xylem remain uncharacterized. PttMC13 and PttMC14 were identified as AtMC9 homologs of hybrid aspen (Populus tremula x tremuloides). A proteomic analysis was conducted in xylem tissues of transgenic hybrid aspen trees which carried either an overexpression or an RNAi construct for PttMC13 and PttMC14. The proteomic analysis revealed modulation of levels of both previously known targets of metacaspases, such as Tudor staphylococcal nuclease, heat shock proteins and 14-3-3 proteins as well as novel targets, such as homologs of the PUTATIVE ASPARTIC PROTEASE 3 (PASPA3) (PASPA) and the cysteine protease RD21 by PttMC13 and PttMC14. We identified here the pathways and processes that are modulated by PttMC13 and PttMC14 in xylem tissues. In particular, the results indicate involvement of PttMC13 and/or PttMC14 in controlling not only xylem cell autolysis but also cell death. This work provides a valuable reference dataset on xylem specific metacaspase functions for future functional and biochemical analyses.
HostingRepository	PRIDE
AnnounceDate	2018-01-02
AnnouncementXML	Submission_2018-01-02_04:00:29.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joakim Bygdell
SpeciesList	scientific name: Populus tremula x Populus tremuloides; NCBI TaxID: 47664;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Synapt G2 HDMS

Revision	Datetime	Status	ChangeLog Entry
0	2017-10-13 01:25:55	ID requested
⏵ 1	2018-01-02 04:00:32	announced

Bollh, ö, ner B, Jokipii-Lukkari S, Bygdell J, Stael S, Adriasola M, Mu, ñ, iz L, Van Breusegem F, Ezcurra I, Wingsle G, Tuominen H, The function of two type II metacaspases in woody tissues of Populus trees. New Phytol, 217(4):1551-1565(2018) [pubmed]

curator keyword: Biological

submitter keyword: Populus, metacaspase, Wood formation, Cell death, LC-MSe

Hannele Tuominen
contact affiliation	Umeå Plant Science Centre, Department of Plant Physiology, Umeå University, 90187 Umeå, Sweden
contact email	hannele.tuominen@umu.se
lab head
Joakim Bygdell
contact affiliation	Computational Life Science Cluster (CLiC), Deptartment of Chemistry, Umeå University
contact email	joakim.bygdell@umu.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/01/PXD007958

PRIDE project URI

• PRIDE
  1. PXD007958
     1. Label: PRIDE project
     2. Name: Populus metacaspases