PXD007955

PXD007955 is an original dataset announced via ProteomeXchange.

Title	Identification of post-translational modifications on Odorant-Binding Protein isoforms from pig olfactory secretome by high-resolution mass spectrometry: O-beta-N-acetylglucosaminylation and phosphorylation
Description	Odorant-Binding Proteins (OBP) are players of perireceptor events in olfaction. A molecular mechanism explaining their specificity for odors and pheromones has still to be proposed. A new track comes from the analysis of pig olfactory secretome, which is mainly composed of OBP isoforms, generated from 3 gene products by PTM, phosphorylation and O‐β‐N‐acetylglucosaminylation (O-GlcNAcylation), which are unusual for secreted proteins. These diverse isoforms could display different binding properties towards ligands. Before testing such a function, both post-translational modifications, although assessed by specific antibodies, have to be identified by mass spectrometry. We report here for the first time, the identification of phosphorylation and O-GlcNAcylation on peptides coming from trypsin digestion of OBP by ESI-HCD-MS/MS. PEAKS software analysis of raw MS data allowed selecting spectra that were analyzed manually to identify PTM. Four peptides corresponding to two different portions of OBP sequence were modified either by a phosphate group or by a hexNAc moiety. Due to the high energy used in HCD, the data did not allow precise localization of the modified sites. We suggest that these two PTM, by generating multiple isoforms, should extend the binding repertoire of secreted OBPs
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_06:51:25.173.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fabrice Bray
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	phosphorylated residue; monohydroxylated residue; complex glycosylation; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2017-10-12 03:27:45	ID requested
1	2018-12-20 00:41:04	announced
⏵ 2	2023-11-14 06:51:25	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: 2-Dimensional Gel Electrophoresis,Odorant-binding protein, O-GlcNAc, ESI-HCD-MS/MS, Phosphorylation

Patricia NAGNAN-LE MEILLOUR
contact affiliation	Glycobiology of olfaction, UMR8576 Unité de glycobiologie structurale et fonctionnelle, France.
contact email	patricia.le-meillour@univ-lille1.fr
lab head
Fabrice Bray
contact affiliation	MSAP3290
contact email	fabrice.bray@univ-lille1.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD007955
     1. Label: PRIDE project
     2. Name: Identification of post-translational modifications on Odorant-Binding Protein isoforms from pig olfactory secretome by high-resolution mass spectrometry: O-beta-N-acetylglucosaminylation and phosphorylation