PXD007949 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | MEERCAT: Multiplexed Efficient Cell Free Expression of Recombinant QconCATS for Large Scale Absolute Proteome Quantification C4PR_LIV |
| Description | A major challenge in proteomics is the absolute accurate quantification of large numbers of proteins. QconCATs, artificial proteins that are concatenations of multiple standard peptides, are well established as an efficient means to generate standards for proteome quantification. Previously, QconCATs have been expressed in bacteria, but we now describe QconCAT expression in a robust, cell-free system. The new expression approach rescues QconCATs that previously were unable to be expressed in bacteria and can reduce the incidence of proteolytic damage to QconCATs. Moreover, it is possible to co-synthesise QconCATs in a highly-multiplexed translation reaction, co-expressing tens or hundreds of QconCATs simultaneously. By obviating bacterial culture and through the gain of high level multiplexing, it is now possible to generate tens of thousands of standard peptides in a matter of weeks, rendering absolute quantification of a complex proteome highly achievable in a reproducible, broadly deployable system. |
| HostingRepository | PRIDE |
| AnnounceDate | 2024-10-22 |
| AnnouncementXML | Submission_2024-10-22_04:41:18.108.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD007949 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Victoria Harman |
| SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
| ModificationList | Label:13C(6)15N(2); Label:13C(6)15N(4); Carbamidomethyl |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2017-10-11 08:38:18 | ID requested | |
| 1 | 2017-10-27 01:05:28 | announced | |
| ⏵ 2 | 2024-10-22 04:41:26 | announced | 2024-10-22: Updated project metadata. |
Publication List
| 10.1074/mcp.ra117.000284; |
| 10.6019/PXD007949; |
| Takemori N, Takemori A, Tanaka Y, Endo Y, Hurst JL, G, รณ, mez-Baena G, Harman VM, Beynon RJ, MEERCAT: Multiplexed Efficient Cell Free Expression of Recombinant QconCATs For Large Scale Absolute Proteome Quantification. Mol Cell Proteomics, 16(12):2169-2183(2017) [pubmed] |
Keyword List
| curator keyword: Technical |
| submitter keyword: QconCAT MEERCAT |
Contact List
| Robert Jeffrey Beynon |
|---|
| contact affiliation | Centre for Proteome Research, Biosciences Building, Institute of Integrative Biology, University of Liverpool, Liverpool, L69 7ZB |
| contact email | rbeynon@liverpool.ac.uk |
| lab head | |
| Victoria Harman |
|---|
| contact affiliation | University of Liverpool |
| contact email | vharman@liverpool.ac.uk |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD007949
- Label: PRIDE project
- Name: MEERCAT: Multiplexed Efficient Cell Free Expression of Recombinant QconCATS for Large Scale Absolute Proteome Quantification C4PR_LIV
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