PXD007942

PXD007942 is an original dataset announced via ProteomeXchange.

Title	Identification of H3 Lysine 4 Monomethylation Associated Proteins at Mammalian Enhancers
Description	Enhancers act to regulate cell type specific gene expression by facilitating the transcription of target genes. In mammalian cells active or primed enhancers are commonly marked by monomethylation of Histone H3 at lysine 4 (H3K4me1) in a cell-type specific manner. Whether and how this histone modification regulates enhancer-dependent transcription programs in mammals has been unclear. In the present study, we conducted SILAC Mass-spec experiments with mono-nucleosomes and identified multiple H3K4me1 associated proteins, including proteins involved in chromatin remodeling. We demonstrate that H3K4me1 augments the association of the chromatin remodeling complex BAF to enhancers in vivo. Furthermore we show that in vitro, H3K4me1 nucleosomes are more efficiently remodeled by the BAF complex. Crystal structures of a BAF component BAF45c further reveal that monomethylation, but not trimethylation, is accommodated in this protein’s H3K4 binding site. Our results suggest that H3K4me1 plays an active role at enhancers by facilitating the binding of the BAF complex and possibly other chromatin regulators.
HostingRepository	PRIDE
AnnounceDate	2018-10-23
AnnouncementXML	Submission_2018-10-23_14:36:18.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Claudio Ponte de Albuquerque
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	carbamoylated residue; 6x(13)C labeled residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2017-10-11 04:02:22	ID requested
⏵ 1	2018-10-23 14:36:19	announced

Local A, Huang H, Albuquerque CP, Singh N, Lee AY, Wang W, Wang C, Hsia JE, Shiau AK, Ge K, Corbett KD, Wang D, Zhou H, Ren B, Identification of H3K4me1-associated proteins at mammalian enhancers. Nat Genet, 50(1):73-82(2018) [pubmed]

curator keyword: Biological

submitter keyword: BAF, monomethylation of Histone H3 at lysine 4 (H3K4me1), and Enhancers

Bing Ren
contact affiliation	Ludwig Institute for Cancer Research
contact email	biren@ucsd.edu
lab head
Claudio Ponte de Albuquerque
contact affiliation	UCSD
contact email	cpontede@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD007942
     1. Label: PRIDE project
     2. Name: Identification of H3 Lysine 4 Monomethylation Associated Proteins at Mammalian Enhancers