PXD007934

PXD007934 is an original dataset announced via ProteomeXchange.

Title	Hyper-specialized Bacteroidetes selfishly compete for laminarin in microalgal blooms
Description	Laminarin is a major storage polysaccharide in phytoplankton and an important carbon and energy source for marine microbes. How microbes compete for this labile polysaccharide in nature remains unclear. Here we investigated metaproteomes and metagenomes of bacterioplankton during four consecutive algal blooms in the North Sea to determine key laminarin consumers. We identified two specialized laminarin degraders of the Bacteroidetes group, which reached high abundances year after year. We found that these genomically streamlined bacteria of the genus Formosa have an expanded set of laminarin hydrolases, sensors and transporters that belonged to the most abundant proteins in the blooms. The respective genes are organized in three polysaccharide utilization loci. Proteomic and biochemical analyses revealed surface tethered enzymes and a laminarinase recombined with a membrane-spanning transporter, which act as a disassembly line and efficiently integrate substrate degradation and uptake in the highly diffusive, aquatic environment. We also show that the bloom bacteria couple laminarin utilization with uptake of cellular building blocks such as amino acids. This study suggests that in addition to genome reduction, enzyme fusions, transporter and enzyme expansion also the tight coupling of the carbon and nitrogen uptake make Formosa spp. efficient laminarin utilizers.
HostingRepository	PRIDE
AnnounceDate	2018-07-31
AnnouncementXML	Submission_2018-08-15_07:50:34.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Frank Unfried
SpeciesList	scientific name: Formosa sp. Hel1_33_131; NCBI TaxID: 1336794;
ModificationList	monohydroxylated residue
Instrument	LTQ Orbitrap Classic

Revision	Datetime	Status	ChangeLog Entry
0	2017-10-10 03:22:39	ID requested
1	2018-07-31 06:18:17	announced
⏵ 2	2018-08-15 07:50:36	announced	Updated publication reference for PubMed record(s): 30061707.

Unfried F, Becker S, Robb CS, Hehemann JH, Markert S, Heiden SE, Hinzke T, Becher D, Reintjes G, Kr, ü, ger K, Avc, ı B, Kappelmann L, Hahnke RL, Fischer T, Harder J, Teeling H, Fuchs B, Barbeyron T, Amann RI, Schweder T, Adaptive mechanisms that provide competitive advantages to marine bacteroidetes during microalgal blooms. ISME J, 12(12):2894-2906(2018) [pubmed]

curator keyword: Biological

submitter keyword: Bacteroidetes, Laminarin degradation, marine, Formosa

Thomas Schweder
contact affiliation	Department of Pharmaceutical Biotechnology, Institute of Pharmacy, University of Greifswald, Germany
contact email	schweder@uni-greifswald.de
lab head
Frank Unfried
contact affiliation	University of Greifswald
contact email	fu072203@uni-greifswald.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD007934
     1. Label: PRIDE project
     2. Name: Hyper-specialized Bacteroidetes selfishly compete for laminarin in microalgal blooms