PXD007933

PXD007933 is an original dataset announced via ProteomeXchange.

Title	Ricin-Like Proteins from the Castor Plant Do Not Influence Liquid Chromatography-Mass Spectrometry Detection of Ricin in Forensically Relevant Samples
Description	The toxic protein ricin (also known as RCA60), found in the seed of the castor plant (Ricinus communis) is frequently encountered in law enforcement investigations. The ability to detect ricin by analyzing its proteolytic (tryptic) peptides by liquid chromatography-tandem mass spectrometry (LC-MS/MS) is well established. However, ricin is just one member of a family of proteins in R. communis with closely related amino acid sequences, including R. communis agglutinin I (RCA120) and other ricin-like proteins (RLPs). Inferring the presence of ricin from its constituent peptides requires an understanding of the specificity, or uniqueness to ricin, of each peptide. Here we describe the set of ricin-derived tryptic peptides that can serve to uniquely identify ricin in distinction to closely-related RLPs and to proteins from other species. Other ricin-derived peptide sequences occur only in the castor plant, and still others are shared with unrelated species. We also characterized the occurrence and relative abundance of ricin and related proteins in an assortment of forensically relevant crude castor seed preparations. We find that whereas ricin and RCA120 are abundant in castor seed extracts, other RLPs are not represented by abundant unique peptides. Therefore, the detection of peptides shared between ricin and RLPs (other than RCA120) in crude castor seed extracts most likely reflects the presence of ricin in the sample.
HostingRepository	PRIDE
AnnounceDate	2017-10-17
AnnouncementXML	Submission_2017-10-17_00:59:07.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Owen Leiser
SpeciesList	scientific name: Ricinus communis (Castor bean); NCBI TaxID: 3988;
ModificationList	monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-10-10 02:08:00	ID requested
⏵ 1	2017-10-17 00:59:07	announced

Dataset with its publication pending

curator keyword: Technical

submitter keyword: Ricin, ricin-like proteins, forensics, LC-MS/MS

Karen Wahl
contact affiliation	Pacific Northwest National Laboratory
contact email	Karen.Wahl@pnnl.gov
lab head
Owen Leiser
contact affiliation	Pacific Northwest National Laboratory
contact email	Owen.Leiser@pnnl.gov
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD007933
     1. Label: PRIDE project
     2. Name: Ricin-Like Proteins from the Castor Plant Do Not Influence Liquid Chromatography-Mass Spectrometry Detection of Ricin in Forensically Relevant Samples