PXD007931 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Comparative proteomics analysis identifies Cdc42-Cdc42BPA signaling as prognostic biomarker and therapeutic target for colon cancer invasion |
Description | Eight rounds of selection of cancer cells invading through matrigel-coated chamber was performed to obtain highly invasive colon cancer sublines HCT116-I8 and RKO-I8. Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) technology was used to identify the differently expressed proteins and the proteomics data was analyzed by ingenuity pathway analysis (IPA). PAK1-PBD immunoprecipitation combined with Western blot were carried out to determine Cdc42 activity, and qRT-PCR and Western blot were used to determine gene expression. The functional role of Cdc42BPA and Cdc42 pathway in colon cancer invasion was studied by loss-of-function experiments including pharmacological blockade, siRNA knockdown, chamber invasion, and WST-1 assays. Human colon cancer tissue microarray was analyzed by immunohistochemistry for overexpression of Cdc42BPA and its correlation with clinicopathological parameters and patient survival outcomes. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:14:40.395.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | huifang hu |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-10-10 01:42:07 | ID requested | |
1 | 2017-11-06 06:28:03 | announced | |
⏵ 2 | 2024-10-22 04:14:41 | announced | 2024-10-22: Updated project metadata. |
Publication List
Hu HF, Xu WW, Wang Y, Zheng CC, Zhang WX, Li B, He QY, Comparative Proteomics Analysis Identifies Cdc42-Cdc42BPA Signaling as Prognostic Biomarker and Therapeutic Target for Colon Cancer Invasion. J Proteome Res, 17(1):265-275(2018) [pubmed] |
10.1021/acs.jproteome.7b00550; |
Keyword List
curator keyword: Biomedical |
submitter keyword: SILAC proteomics,Cdc42, Cdc42BPA, colon cancer invasion, prognostic biomarker, targeted therapy |
Contact List
Dr. Bin Li |
contact affiliation | Institute of Life and Health Engineering, College of Life Science and Technology, Jinan University, |
contact email | libin2015@jnu.edu.cn |
lab head | |
huifang hu |
contact affiliation | 暨南大学 |
contact email | 1282096367@qq.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD007931
- Label: PRIDE project
- Name: Comparative proteomics analysis identifies Cdc42-Cdc42BPA signaling as prognostic biomarker and therapeutic target for colon cancer invasion