PXD007931

PXD007931 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Comparative proteomics analysis identifies Cdc42-Cdc42BPA signaling as prognostic biomarker and therapeutic target for colon cancer invasion
Description	Eight rounds of selection of cancer cells invading through matrigel-coated chamber was performed to obtain highly invasive colon cancer sublines HCT116-I8 and RKO-I8. Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) technology was used to identify the differently expressed proteins and the proteomics data was analyzed by ingenuity pathway analysis (IPA). PAK1-PBD immunoprecipitation combined with Western blot were carried out to determine Cdc42 activity, and qRT-PCR and Western blot were used to determine gene expression. The functional role of Cdc42BPA and Cdc42 pathway in colon cancer invasion was studied by loss-of-function experiments including pharmacological blockade, siRNA knockdown, chamber invasion, and WST-1 assays. Human colon cancer tissue microarray was analyzed by immunohistochemistry for overexpression of Cdc42BPA and its correlation with clinicopathological parameters and patient survival outcomes.
HostingRepository	PRIDE
AnnounceDate	2017-11-06
AnnouncementXML	Submission_2017-11-06_06:28:02.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	huifang hu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2017-10-10 01:42:07	ID requested
⏵ 1	2017-11-06 06:28:03	announced

Publication List

Hu HF, Xu WW, Wang Y, Zheng CC, Zhang WX, Li B, He QY, Comparative Proteomics Analysis Identifies Cdc42-Cdc42BPA Signaling as Prognostic Biomarker and Therapeutic Target for Colon Cancer Invasion. J Proteome Res, 17(1):265-275(2018) [pubmed]

Hu HF, Xu WW, Wang Y, Zheng CC, Zhang WX, Li B, He QY, Comparative Proteomics Analysis Identifies Cdc42-Cdc42BPA Signaling as Prognostic Biomarker and Therapeutic Target for Colon Cancer Invasion. J Proteome Res, 17(1):265-275(2018) [pubmed]

Keyword List

curator keyword: Biomedical
submitter keyword: SILAC proteomics，Cdc42, Cdc42BPA, colon cancer invasion, prognostic biomarker, targeted therapy

curator keyword: Biomedical

submitter keyword: SILAC proteomics，Cdc42, Cdc42BPA, colon cancer invasion, prognostic biomarker, targeted therapy

Contact List

Dr. Bin Li
contact affiliation	Institute of Life and Health Engineering, College of Life Science and Technology, Jinan University,
contact email	libin2015@jnu.edu.cn
lab head
huifang hu
contact affiliation	暨南大学
contact email	1282096367@qq.com
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007931
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007931

PRIDE project URI

Repository Record List

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