PXD007928

PXD007928 is an original dataset announced via ProteomeXchange.

Title	Cross-linking of the human MHC-I peptide-loading complex
Description	The peptide-loading complex (PLC) is a transient, multisubunit membrane complex in the endoplasmic reticulum (ER), essential for establishing a hierarchical immune response. The PLC coordinates peptide translocation into the ER with loading and editing of major histocompatibility complex class I molecules (MHC-I). After final proofreading in the PLC, stable peptide/MHC-I complexes are released to the cell surface to evoke a T-cell response against infected or malignant cells. Sampling of different MHC-I allomorphs requires the precise coordination of seven different subunits into a single macromolecular assembly, including the transporter associated with antigen processing TAP1/2, the oxidoreductase ERp57, the MHC-I heterodimer, and the chaperones tapasin and calreticulin. The molecular organization and mechanistic events in the PLC are unknown due to the compositional heterogeneity and intrinsic dynamic nature of the complex. Here, PLC from Burkitt’s lymphoma cells was isolated using an engineered viral inhibitor as bait, followed by cross-linking mass spectrometry to aid EM structure elucidation.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:14:52.493.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tommy Hofmann
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	methionine oxidation with neutral loss of 64 Da; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-10-09 05:39:23	ID requested
1	2017-11-07 04:05:48	announced
⏵ 2	2024-10-22 04:14:58	announced	2024-10-22: Updated project metadata.

Blees A, Januliene D, Hofmann T, Koller N, Schmidt C, Trowitzsch S, Moeller A, Tamp, é R, Structure of the human MHC-I peptide-loading complex. Nature, 551(7681):525-528(2017) [pubmed]

curator keyword: Biological

submitter keyword: cross-linking, cryo-electron microscopy, structure, MHC-I peptide-loading complex

Carla Schmidt
contact affiliation	Interdisciplinary research center HALOmem, Martin Luther University Halle-Wittenberg, Kurt-Moses-Straße-3, 06120 Halle (Saale)
contact email	Carla.Schmidt@biochemtech.uni-halle.de
lab head
Tommy Hofmann
contact affiliation	Interdisciplinary research center HALOmem, Martin Luther University Halle-Wittenberg, Kurt-Moses-Straße-3, 06120 Halle (Saale
contact email	Tommy.Hofmann@biochemtech.uni-halle.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD007928
     1. Label: PRIDE project
     2. Name: Cross-linking of the human MHC-I peptide-loading complex