PXD007910

PXD007910 is an original dataset announced via ProteomeXchange.

Title	The mitogen-activated protein kinase kinase 5 regulates the proliferation and biosynthetic processes in procyclic forms of Trypanosoma brucei
Description	The pathogenic protozoan T. brucei alternates into distinct developmental stages in the mammalian and insect hosts. The mitogen-activated protein kinase (MAPK) signaling pathways transduce extracellular stimuli into a range of cellular responses, which ultimately lead to the adaptation to the external environment. Here, we combined a loss of function approach with stable isotope labelling with amino acids in cell culture (SILAC)-based mass spectrometry (MS) to investigate the role of the mitogen-activated kinase kinase 5 (MKK5) in T. brucei. The silencing of MKK5 significantly decreased the proliferation of procyclic forms of T. brucei. To shed light into the molecular alterations associated with this phenotype, we measured the total proteome and phosphoproteome of cells silenced for MKK5. In the total proteome, we observed a general decrease in proteins related to ribosome and translation as well as down-regulation of several components of the fatty acid biosynthesis pathway. In addition, we observed alterations in the protein levels and phosphorylation of key metabolic enzymes, which point toward a suppression of the oxidative metabolism. Taken together, our findings show that the silencing of MKK5 alters cell growth, energy metabolism, protein and fatty acid biosynthesis in procyclic T. brucei.
HostingRepository	PRIDE
AnnounceDate	2017-10-20
AnnouncementXML	Submission_2017-10-20_02:20:10.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fernanda Kugeratski
SpeciesList	scientific name: Trypanosoma brucei; NCBI TaxID: 5691;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2017-10-09 01:37:51	ID requested
⏵ 1	2017-10-20 02:20:11	announced

Kugeratski FG, Batista M, Lima CVP, Neilson LJ, da Cunha ES, de Godoy LM, Zanivan S, Krieger MA, Marchini FK, Mitogen-Activated Protein Kinase Kinase 5 Regulates Proliferation and Biosynthetic Processes in Procyclic Forms of Trypanosoma brucei. J Proteome Res, 17(1):108-118(2018) [pubmed]

curator keyword: Biological

submitter keyword: mitogen-activated protein kinase kinase 5 (MKK5), T. brucei , proteomics, phosphoproteomics, energy metabolism, fatty acids biosynthesis

Fabricio Marchini
contact affiliation	Carlos Chagas Institute, Fiocruz Parana, Functional Genomics Laboratory, Brazil Carlos Chagas Institute, Fiocruz Parana, Mass Spectrometry Facility RPT02H, Brazil
contact email	fabricio.marchini@fiocruz.br
lab head
Fernanda Kugeratski
contact affiliation	CRUK Beatson Institute
contact email	fernanda.kugeratski@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/10/PXD007910

PRIDE project URI

• PRIDE
  1. PXD007910
     1. Label: PRIDE project
     2. Name: The mitogen-activated protein kinase kinase 5 regulates the proliferation and biosynthetic processes in procyclic forms of Trypanosoma brucei