PXD007907

PXD007907 is an original dataset announced via ProteomeXchange.

Title	Tef (Eragrostis tef) iTRAQ-labelled protein fractions
Description	The orphan crop, Eragrostis tef, was subjected to controlled drought conditions to observe the physiological parameters and proteins changing in response to dehydration stress. Physiological measurements involving electrolyte leakage, chlorophyll fluorescence and ultra-structural analysis showed tef plants to tolerate water loss to 50% RWC before adverse effects in leaf tissues were observed. Proteomic analysis using iTRAQ mass spectrometry and appropriate database searching enabled the detection of 5727 proteins, of which 211 proteins, including a number of spliced variants, were found to be differentially regulated with imposed stress conditions. Validation of the stress-related proteins, fructose-bisphosphate aldolase (FBA), glutamine synthetase (GLN) and the protective antioxidant proteins, monodehydroascorbate reductase (MDHAR) and peroxidase (POX), using immunodetection and enzymatic assay confirmed protein presence according to iTRAQ findings and showed increased protein abundance levels and enzymatic activity in response to water-deficit. GO-term enrichment and analysis revealed terms involved in biotic and abiotic stress response, signaling, transport, cellular homeostasis and pentose metabolic processes, to be enriched in tef up-regulated proteins, while terms linked to ROS-producing processes under water-deficit, such as photosynthesis and associated light harvesting reactions, manganese transport and homeostasis, the synthesis of sugars and cell wall catabolism and modification, to be enriched in tef down-regulated proteins.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:14:34.351.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rizqah Kamies
SpeciesList	scientific name: Oryza sativa (Rice); NCBI TaxID: 4530; scientific name: Zea mays (Maize); NCBI TaxID: 4577; scientific name: Hordeum vulgare (Barley); NCBI TaxID: 4513;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue
Instrument	6520A Quadrupole Time-of-Flight LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2017-10-05 01:41:36	ID requested
1	2017-11-21 05:27:17	announced
⏵ 2	2024-10-22 04:14:41	announced	2024-10-22: Updated project metadata.

Kamies R, Farrant JM, Tadele Z, Cannarozzi G, Rafudeen MS, A Proteomic Approach to Investigate the Drought Response in the Orphan Crop Eragrostis tef. Proteomes, 5(4):(2017) [pubmed]

10.3390/proteomes5040032;

curator keyword: Biological

submitter keyword: tef, OFFGEL fractionation, dehydration stress, quantitative proteomics, iTRAQ, total proteins

Dr Suhail Rafudeen
contact affiliation	Plant Stress Lab, Department of Molecular and Cell Biology, University of Cape Town
contact email	suhail.rafudeen@uct.ac.za
lab head
Rizqah Kamies
contact affiliation	University of Cape Town
contact email	rizqahkamies@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007907

PRIDE project URI

• PRIDE
  1. PXD007907
     1. Label: PRIDE project
     2. Name: Tef (Eragrostis tef) iTRAQ-labelled protein fractions