PXD007902

PXD007902 is an original dataset announced via ProteomeXchange.

Title	Myristic acid induces proteomic and secretomic changes associated with steatosis, cytoskeleton remodeling, endoplasmatic reticulum stress and exosome release in HepG2 cells
Description	Myristic acid, the 14-carbon saturated fatty acid (C14:0), is usually associated with negative consequences for human health, and in particular its consumption is correlated to an increased cardiovascular disease risk. Since it is a little abundant into the cells, its specific properties and functional roles have not been fully described. The aim of this study was to explore the cell response to this fatty acid to help explain clinical findings on the relationship between C14:0 and cardiovascular disease. The human liver HepG2 cell line was used as a model to investigate the hepatic response to C14:0 in a combined proteomic and secretomic approach. A total of 47 intracellular and 32 secreted proteins were found to be deregulated among the different dosages of C14:0 treatments (50, 125, and 250 μM) as compared to control. Bioinformatics analysis revealed that myristic acid modulates the lipid droplet formation and the cytoskeleton organization, in addition it induces ER stress as well as changes in exosome and extracellular miRNA sorting. Our data provide for the first time quantitative proteomic data regarding C14:0 and contribute to the explanation of the molecular mechanisms through which this fatty acid can cause adverse health effects.
HostingRepository	PRIDE
AnnounceDate	2018-04-17
AnnouncementXML	Submission_2018-04-17_00:15:05.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Barbara Darnhofer
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Bruker Daltonics maXis series

Revision	Datetime	Status	ChangeLog Entry
0	2017-10-04 02:39:38	ID requested
⏵ 1	2018-04-17 00:15:06	announced

Speziali G, Liesinger L, Gindlhuber J, Leopold C, Pucher B, Brandi J, Castagna A, Tomin T, Krenn P, Thallinger GG, Olivieri O, Martinelli N, Kratky D, Schittmayer M, Birner-Gruenberger R, Cecconi D, Myristic acid induces proteomic and secretomic changes associated with steatosis, cytoskeleton remodeling, endoplasmic reticulum stress, protein turnover and exosome release in HepG2 cells. J Proteomics, 181():118-130(2018) [pubmed]

curator keyword: Biological

submitter keyword: Human, LC-MS/MS, Myristic acid, HepG2

Ruth Birner-Gruenberger
contact affiliation	Austrian Centre of Industrial Biotechnology (ACIB), Petersgasse 14, 8010 Graz, Austria; Medical University of Graz, Institute of Pathology, Research Unit Functional Proteomics and Metabolic Pathways, Stiftingtalstrasse 24, 8010 Graz, Austria; Omics Center Graz, BioTechMed-Graz, Stiftingtalstrasse 24, 8010 Graz, Austria.
contact email	ruth.birner-gruenberger@medunigraz.at
lab head
Barbara Darnhofer
contact affiliation	Omics Center Graz
contact email	barbara.darnhofer@klinikum-graz.at
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD007902
     1. Label: PRIDE project
     2. Name: Myristic acid induces proteomic and secretomic changes associated with steatosis, cytoskeleton remodeling, endoplasmatic reticulum stress and exosome release in HepG2 cells