PXD007888

PXD007888 is an original dataset announced via ProteomeXchange.

Title	HAX1-interactome in breast cancer cell line (MCF7)
Description	The MCF7 cell line represents a typical epithelial cell line and corresponds to luminal A breast cancer (estrogen-responsive). Overexpression of HAX1 was demonstrated in MCF7 cell line as well as in breast cancer samples, suggesting a role of HAX1 in breast cancer progression. HAX1 is a 32-kDa protein of unknown structure, involved in the regulation of apoptosis, cell migration and calcium homeostasis. It was also shown to bind mRNA. Scarcity of structural elements and the presence of a disordered region, inferred from HAX1 sequence, suggests that HAX1 is intrinsically disordered, and may have many protein-protein interactions. So far about 40 different proteins were characterized as HAX1 protein partners. In the present work, applying immunoaffinity chromatography coupled with mass spectrometry, we identified new candidates for HAX1 binding partners in breast cancer cells. Newly identified proteins may be divided into three, partially overlapping groups: cytoskeleton-associated proteins, GTP-ase associated proteins and RNA-binding proteins. These results imply that HAX1 has more protein partners than hitherto described. Subsequent analysis of these interactions may shed some light into molecular mechanisms of HAX1 functions.
HostingRepository	PRIDE
AnnounceDate	2019-11-14
AnnouncementXML	Submission_2020-11-11_09:11:41.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD007888
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Tymon Rubel
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	methylthiolated residue; monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-10-02 02:54:48	ID requested
1	2019-11-13 23:28:54	announced
⏵ 2	2020-11-11 09:11:43	announced	2020-11-11: Updated publication reference for PubMed record(s): 31644363, 33146709.

Balcerak A, Trebinska-Stryjewska A, Wakula M, Chmielarczyk M, Smietanka U, Rubel T, Konopinski R, Macech-Klicka E, Zub R, Grzybowska EA, HAX1 impact on collective cell migration, cell adhesion, and cell shape is linked to the regulation of actomyosin contractility. Mol Biol Cell, 30(25):3024-3036(2019) [pubmed]

Wakula M, Balcerak A, Rubel T, Chmielarczyk M, Konopinski R, Lyczek F, Grzybowska EA, The interactome of multifunctional HAX1 protein suggests its role in the regulation of energy metabolism, de-aggregation, cytoskeleton organization and RNA-processing. Biosci Rep, 40(11):(2020) [pubmed]

curator keyword: Biological

submitter keyword: HAX1, interactome, MCF7

Ewa Grzybowska
contact affiliation	Department of Molecular and Translational Oncology, Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology; Warsaw, Poland
contact email	Ewa.Grzybowska@coi.pl
lab head
Tymon Rubel
contact affiliation	Institute of Radioelectronics and Multimedia Technology Warsaw University of Technology
contact email	trubel@ire.pw.edu.pl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD007888

PRIDE project URI

• PRIDE
  1. PXD007888
     1. Label: PRIDE project
     2. Name: HAX1-interactome in breast cancer cell line (MCF7)