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PXD007887

PXD007887 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHAX1-interactome in cervical cancer cell line (HeLa)
DescriptionHAX1 is a 32-kDa protein of unknown structure, involved in the regulation of apoptosis, cell migration and calcium homeostasis. It was also shown to bind mRNA. Scarcity of structural elements and the presence of a disordered region, inferred from HAX1 sequence, suggests that HAX1 is intrinsically disordered, and may have many protein-protein interactions. So far about 40 different proteins were characterized as HAX1 protein partners. In the present work, applying immunoaffinity chromatography coupled with mass spectrometry, we identified new candidates for HAX1 binding partners in cervical carcinoma cells. More than a half of these newly identified proteins represent RNA-binding proteins. There is also a big group of proteins, mostly mitochondrial, involved in cellular metabolism. These results imply that HAX1 has more protein partners than hitherto described. Subsequent analysis of these interactions may shed some light into molecular mechanisms of HAX1 functions, especially its involvement in mRNA processing and its role in protection against oxidative stress.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:14:57.189.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD007887
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterTymon Rubel
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmethylthiolated residue; monohydroxylated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-10-02 02:47:11ID requested
12020-11-11 09:13:52announced
22024-10-22 05:14:58announced2024-10-22: Updated project metadata.
Publication List
10.1042/bsr20203094;
Wakula M, Balcerak A, Rubel T, Chmielarczyk M, Konopinski R, Lyczek F, Grzybowska EA, The interactome of multifunctional HAX1 protein suggests its role in the regulation of energy metabolism, de-aggregation, cytoskeleton organization and RNA-processing. Biosci Rep, 40(11):(2020) [pubmed]
10.6019/PXD007887;
Keyword List
curator keyword: Biological
submitter keyword: HAX1, HeLa, interactome
Contact List
Ewa Grzybowska
contact affiliationDepartment of Molecular and Translational Oncology, Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology; Warsaw, Poland
contact emailEwa.Grzybowska@coi.pl
lab head
Tymon Rubel
contact affiliationInstitute of Radioelectronics and Multimedia Technology Warsaw University of Technology
contact emailtrubel@ire.pw.edu.pl
dataset submitter
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Dataset FTP location
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PRIDE project URI
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