PXD007887 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | HAX1-interactome in cervical cancer cell line (HeLa) |
Description | HAX1 is a 32-kDa protein of unknown structure, involved in the regulation of apoptosis, cell migration and calcium homeostasis. It was also shown to bind mRNA. Scarcity of structural elements and the presence of a disordered region, inferred from HAX1 sequence, suggests that HAX1 is intrinsically disordered, and may have many protein-protein interactions. So far about 40 different proteins were characterized as HAX1 protein partners. In the present work, applying immunoaffinity chromatography coupled with mass spectrometry, we identified new candidates for HAX1 binding partners in cervical carcinoma cells. More than a half of these newly identified proteins represent RNA-binding proteins. There is also a big group of proteins, mostly mitochondrial, involved in cellular metabolism. These results imply that HAX1 has more protein partners than hitherto described. Subsequent analysis of these interactions may shed some light into molecular mechanisms of HAX1 functions, especially its involvement in mRNA processing and its role in protection against oxidative stress. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:14:57.189.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD007887 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Tymon Rubel |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | methylthiolated residue; monohydroxylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-10-02 02:47:11 | ID requested | |
1 | 2020-11-11 09:13:52 | announced | |
⏵ 2 | 2024-10-22 05:14:58 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1042/bsr20203094; |
Wakula M, Balcerak A, Rubel T, Chmielarczyk M, Konopinski R, Lyczek F, Grzybowska EA, The interactome of multifunctional HAX1 protein suggests its role in the regulation of energy metabolism, de-aggregation, cytoskeleton organization and RNA-processing. Biosci Rep, 40(11):(2020) [pubmed] |
10.6019/PXD007887; |
Keyword List
curator keyword: Biological |
submitter keyword: HAX1, HeLa, interactome |
Contact List
Ewa Grzybowska |
contact affiliation | Department of Molecular and Translational Oncology, Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology; Warsaw, Poland |
contact email | Ewa.Grzybowska@coi.pl |
lab head | |
Tymon Rubel |
contact affiliation | Institute of Radioelectronics and Multimedia Technology Warsaw University of Technology |
contact email | trubel@ire.pw.edu.pl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD007887
- Label: PRIDE project
- Name: HAX1-interactome in cervical cancer cell line (HeLa)