PXD007885

PXD007885 is an original dataset announced via ProteomeXchange.

Title	Analysis of secreted S. aureus proteins after isolation of phagosomes from infected human epithelial cells
Description	Bacteria such as Staphylococcus aureus are generally engulfed by the host with a membrane when internalized by non-professional phagocytic host cells. These vacuoles then mature to phagosomes which can fuse with lysosomes enabling killing and digestion of the pathogen. Former proteome approaches enriching S. aureus from lysed host cells after infection did not cover secreted virulence factors. However, secreted S. aureus proteins should be trapped within the phagosomes and, hence, these compartments were enriched from human bronchial epithelial S9 cells infected with S. aureus HG001 to also explore the virulence factor repertoire of S. aureus following internalization by host cells. Using shotgun mass spectrometry we monitored 92 phagosomal proteins over time unraveling a heterogeneous composition of phagosomal proteins in regard to maturation stages. Furthermore, we quantified 36 bacterial proteins within the phagosomes during the first 6.5 h post infection by applying targeted single reaction monitoring. Among them were secreted bacterial virulence factors like lipases, hemolysins, and secreted adhesins which are usually hard to detect from infected sample material by proteomics approaches due to low abundance. Thus, we provide insight into the fate and early adaptation of S. aureus HG001 after internalization by epithelial cells by simultaneous analysis of the phagosomal environment and virulence factors of S. aureus.
HostingRepository	PRIDE
AnnounceDate	2018-06-29
AnnouncementXML	Submission_2018-06-28_23:44:02.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kristin Surmann
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	monohydroxylated residue; iodoacetic acid derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-10-02 02:35:38	ID requested
⏵ 1	2018-06-28 23:44:03	announced

Surmann K, Depke M, Dhople VM, Pan, é, -Farr, é J, Hildebrandt P, Gumz J, Schaible UE, V, ö, lker U, Schmidt F, Analysis of Staphylococcus aureus proteins secreted inside infected human epithelial cells. Int J Med Microbiol, 308(6):664-674(2018) [pubmed]

curator keyword: Biological

submitter keyword: host-pathogen-interaction, phagosomes, quantitative proteomics, single reaction monitoring (SRM), Staphylococcus aureus, virulence factors

Frank Schmidt
contact affiliation	Functional Genomics, University Medicine Greifswald, Germany
contact email	frank.schmidt@uni-greifswald.de
lab head
Kristin Surmann
contact affiliation	University Medicine Greifswald
contact email	surmannk@uni-greifswald.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD007885

PRIDE project URI

• PRIDE
  1. PXD007885
     1. Label: PRIDE project
     2. Name: Analysis of secreted S. aureus proteins after isolation of phagosomes from infected human epithelial cells